牛磺酸对急性染锰大鼠空间学习记忆能力的改善作用  

Effects of taurine on spatial memory of rats exposed to manganese chloride

作　　者：陆彩玲[1] 吴元桢[1] 唐付华[1] 刘楠楠[1] 黄玲[1] 郭松超[1] 

机构地区：[1]广西医科大学公共卫生学院营养与食品卫生学教研室,广西南宁530021

出　　处：《中国药理学与毒理学杂志》2011年第3期254-257,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金课题(30660156);广西研究生教育创新计划(2009105981004D24)~~

摘　　要：目的探讨急性染锰对大鼠学习记忆能力的影响及牛磺酸的干预作用。方法 (1)牛磺酸预防实验:染锰组大鼠每日ip给予MnCl2.4H2O15 mg·kg-1,连续4周。染锰+牛磺酸预防组大鼠染锰的同时ip牛磺酸200 mg·kg-1,持续4周。(2)牛磺酸治疗实验:每日ip给予MnCl2.4H2O15 mg·kg-1染锰,4周后再ip牛磺酸200 mg·kg-1,持续4周。水迷宫实验检测逃避潜伏时间及平台搜索次数。分离大鼠海马组织并测定乙酰胆碱酯酶(AChE)活力及胆碱O-乙酰转移酶(ChAT)活力。结果 (1)牛磺酸预防实验:与正常对照组逃避潜伏时间(29.5±2.5)s相比,染锰对照组明显延长为(39.8±2.3)s,与染锰对照组相比,牛磺酸预防组逃避潜伏时间明显缩短为(29.4±2.3)s(P<0.05)。与正常对照组相比,染锰对照组海马组织AChE活力无显著性差异,但牛磺酸预防组酶活力则显著下降(P<0.05)。三组间ChAT活力无明显差异。(2)牛磺酸治疗实验:与染锰对照组逃避潜伏时间(56.6±3.0)s相比,牛磺酸治疗组显著缩短为(27.8±2.3)s(P<0.05),平台搜索次数无显著性差异。与染锰对照组AChE和ChAT活力显著增加(P<0.05)。结论牛磺酸预防或治疗可明显改善急性染锰诱导大鼠空间学习记忆能力下降,其机制可能与海马内乙酰胆碱含量有关。OBJECTIVE To explore the effect of taurine on spatial memory of rats exposed to manganese chloride（MnCl2）.METHODS（1） Pretreatment experiment： the rats were ip given taurine 200 mg·kg^-1 together with MnCl2·4H2O 15 mg·kg^-1 once daily for 4 weeks.（2） Taurine treatment experiment： the rats in taurine treatment group were ip given taurine 200 mg·kg^-1 for another 4 weeks after given MnCl2·4H2O 15 mg·kg^-1 for 4 weeks.The spatial learning ability was evaluated with the average escape latency to the platform when rats were placed randomly into the swimming pool on the first 5 d.The spatial memory ability was evaluated by the times crossing the target platform on the sixth day.Hippocampus was collected to detect the activity of acetylcholine esterase（AChE） and choline O-acetyltransferase（ChAT）.RESULTS（1） Pretreatment experiment： compared with normal control group（29.5±2.5）s,the escape latency in MnCl2 exposed group markedly prolonged to（39.8±2.3）s（P0.05）,compared with MnCl2 exposure group,the escape latency in taurine pretment group significantly decreased to（29.4±2.3）s（P0.05）.There was no significant difference in AChE and ChAT activities between normal control and MnCl2 exposure group.Pretreatment with taurine could decrease AChE activity significantly compared with MnCl2 exposure group（P0.05）.（2） Taurine treatment experiment： there was no significant difference in escapy latency and the times crossing the target platform between MnCl2 exposure group and taurine treatment group.The activities of AChE and ChAT were increased in taurine group compared with MnCl2 exposure group（P0.05）.CONCLUSION Taurine can improve the impaired spatial learning ability induced by MnCl2,possibly related to the acetylcholine content.

关 键 词：锰 牛磺酸 学习 记忆障碍 乙酰胆碱 

分 类 号：R964[医药卫生—药理学] R977.4[医药卫生—药学]