INF-γ第三内含子区+3586位点基因多态性与多发性硬化相关性的研究  

Relationship between the polymorphism in the third intron+3586 of interferon-γ gene and susceptibility to multiple sclerosis

作　　者：王秀丽[1] 李亚[2] 张牧霞[2] 孟凡茹[1] 王鑫[1] 郭力[3] 

机构地区：[1]河北医科大学第二医院检验科,河北石家庄050000 [2]河北医科大学第三医院实验中心,河北石家庄050000 [3]河北医科大学第二医院神经内科,河北石家庄050000

出　　处：《中风与神经疾病杂志》2011年第6期538-540,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究干扰素-γ(interferon gamma,IFN-γ)第三内含子区+3586位点单核苷酸多态性与多发性硬化(multiple sclerosis,MS)易感性的相关性。方法选取河北医科大学第二医院住院多发性硬化患者58例,正常对照40例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法对INF-γ第三内含子+3586位点单核苷酸多态性进行分析,经统计学处理,研究其与多发性硬化易感性的相关性。结果 IFN-γ+3586位点MS组与健康对照组基因型分布的比较差异没有统计学意义(P>0.05);MS组IFN-γ+3586位点A及G等位基因频率与健康对照组比较均没有统计学意义(P>0.05)。结论 IFN-γ第三内含子区+3586位点基因多态性与MS的遗传易感性不相关。Objective To study the association between polymorphism in the third intron of IFN-γ gene and the susceptibility to MS.Methods A population-based case-control study was carried out.58 patients with MS and 40 healthy controls were interviewed.Meanwhile,the genetic polymorphism of INF-γ intron Ⅲ ＋3586A allele was detected using polymerase chain reaction-restriction fragment length polymorphism.Results No significant difference of genotypes and site frequencies of INF-γ intron Ⅲ ＋3586 existed in MS group compared with in the control group（P0.05）.Conclusion The INF-γ intron Ⅲ（＋3586） gene polymorphism might not be associated with MS genetic susceptibility.

关 键 词：多发性硬化 干扰素-Γ 第三内含子 基因多态性 基因型 

分 类 号：R744.51[医药卫生—神经病学与精神病学]