神经病理性痛大鼠背根神经节和脊髓背角Nogo—A蛋白表达的变化  

Changes in expression of Nogo-A protein in dorsal root ganglion and spinal dorsal horn in a rat model of neuropathic pain

作　　者：许丹阳[1] 褚智幸[1] 袁燕平[1] 张励才[1] 

机构地区：[1]江苏省徐州医学院麻醉学重点实验室,221002

出　　处：《中华麻醉学杂志》2011年第4期439-441,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（NSFC30570974）;徐州医学院研究生科技创新计划基金

摘　　要：目的评价神经病理性痛大鼠背根神经节和脊髓背角Nogo—A蛋白表达的变化。方法健康成年雄性SD大鼠72只，体重250～300g，采用随机数字表法，将大鼠随机分为3组（n=24）：对照组（C组）、假手术组（S组）和神经病理性痛组（NP组）。C组不做任何处理，NP组采用结扎并剪断胫神经和腓总神经的方法制备大鼠神经病理性痛模型，S组仅切皮暴露坐骨神经但不结扎和剪断神经。于结扎后1、7、14、21d时采用von Frey丝测定大鼠机械痛阈。于各时点随机取6只大鼠处死后取损伤侧L5背根神经节和L4,5，脊髓，采用免疫荧光标记法测定Nogo—A蛋白的表达（n=3），采用Western blot法测定Nogo-A蛋白的表达（n=3）。结果与C组和S组比较，NP组大鼠结扎后7、14、21d时机械痛阈降低，结扎后7、14d时背根神经节Nogo—A蛋白表达下调，结扎后14、21d时脊髓背角Nogo-A蛋白表达上调（P〈0．05）。结论背根神经节及脊髓背角Nogo-A蛋白可能在外周神经损伤诱发大鼠神经病理性痛过程中发挥重要作用。Objective To investigate the changes in the expression of Nogo-A protein in the dorsal root ganglion （DRG） and spinal dorsal horn in a rat model of neuropathic pain （NP）. Methods Seventy-two male SD rats weighing 250-300 g were randomly divided into 3 groups （ n = 24 each） ： control group （group C）, sham operation group （group S） and NP group. NP was induced by ligation and severance of tibial and common fibular nerves according to the technique described by Isabetle et al. The mechanical withdrawal threshold （MWT） to yon Frey filament stimulation was measured at 1, 7, 14 and 21 days after ligation. Six rats in each group were randomly selected at each time point and sacrificed （3 for determination of Nogo-A protein expression by immunofluorescence, 3 for determination of Nogo-A protein expression by Western blot）. The L5 DRG and L4.5 segment of spinal cord on the injured side were removed for determination of Nogo-A protein expression by Western blot. Results Compared with the groups C and S, MWT was significantly decreased at 7, 14 and 21 days after ligation, the expression of Nogo-A protein in the DRG was down-regulated at 7 and 14 days after ligation and the expression of Nogo-A protein in the spinal dorsal horn was up-regulated at 14 and 21 days after ligation （ P 〈 0.05） .Conclusion The Nogo-A protein in the DRG and spinal dorsal horn may play an important role in peripheral nerve injury-induced NP in rats.

关 键 词：神经痛 脊髓 神经节 脊 NOGO-A蛋白 

分 类 号：R741[医药卫生—神经病学与精神病学]