脊髓小胶质细胞活化在大鼠术后持续性痛中的作用  

The role of microglial activation in spinal cord in a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction

作　　者：尹显和[1] 胡兴国[2] 曾因明[1] 

机构地区：[1]徐州医学院江苏省麻醉与镇痛应用技术重点实验室江苏省麻醉学重点实验室,221002 [2]湖南省桃源县人民医院麻醉科

出　　处：《中华麻醉学杂志》2011年第4期442-445,共4页Chinese Journal of Anesthesiology

基　　金：湖南省科技计划一般项目资助课题（2009SK3018）

摘　　要：目的探讨脊髓小胶质细胞活化在大鼠术后持续性痛中的作用。方法选择鞘内置管成功的雄性SD大鼠72只，体重200—250g，采用随机数字表法，将其随机分为3组（n=24）：假手术组、皮肤肌肉切口牵拉组（SMIR组）和米诺环素组。采用皮肤肌肉切口牵拉法建立大鼠术后持续性痛模型，假手术组和SMIR组于术前30min和术后1～3d鞘内注射人工脑脊液20m，1次／d；米诺环素组于术前30min和术后1—3d鞘内注射米诺环素10出，1次，d，并在每次注药后经导管注射人工脑脊液10m冲洗导管。于术前1d及术后3、7、12、22和32d时测定机械缩足反射阈值（MWT），各时点MWT测定结束后，随机取4只大鼠，测定脊髓背角小胶质细胞特异性标记物Iba-1的表达和计数小胶质细胞。结果与假手术组比较，SMIR组和米诺环素组术后3～22d时MWT降低，术后3、7d时脊髓背角Iba-1表达和小胶质细胞计数升高（P〈0．05）；与SMIR组比较，米诺环素组术后3～22d时MWT升高，术后3、7d时脊髓背角Iba-1表达和小胶质细胞计数降低（P〈0．05）。结论脊髓小胶质细胞的活化参与了大鼠术后持续性痛的形成。Objective To investigate the role of microglial activation in spinal cord in a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction （SMIR）. Methods Seventy-two male SD rats weighing 200-250 g in which intrathecal （IT） catheter was successfully inserted were randomly divided into 3 groups （ n = 24 each） ： group sham operation; group SMIR and group SMIR ＋ IT minocycline （a specific microglia inhibitor）. The rat model of persistent postoperative pain evoked by SMIR was established according to the method described by Flatters. Pain behavior was assessed by paw mechanical withdrawal threshold （MWT） to yon Frey illament stimulation at 1 day before （To ,baseline） and 3, 7, 12, 22 and 32 days after operation （T1-5）. Four animals were sacrificed at each time point in each group for detection of the expression of Iba-1 （a specific marker of microglia） in the spinal dorsal horn by immunofluorescence and the microglia was counted. Results MWT was significantly decreasedat T1-4, while the expression of Iba-1 and microglia counts in the spinal dorsal horn were significantly increased at T1,2 by SMIR in group Ⅱ . IT minocycline significantly attenuated the hyperalgesia induced by SMIR at T1-4 and decreased Iba-1 expression and microglia counts at T1,2 in group m. Conclusion Microglial activation in the spinal cord plays an important role in the development and maintenance of SMIR-evoked persistent postoperative pare in rats.

关 键 词：小神经胶质细胞 疼痛 手术后 脊髓 

分 类 号：R363[医药卫生—病理学]