垂体瘤转化基因小干扰RNA对人皮肤鳞状细胞癌裸鼠移植瘤中基质金属蛋白酶2和9表达的影响  

Effect of PTTG siRNA on MMP-2 and MMP-9 expression in nude mice bearing xenografted human cutaneous squamous cell carcinoma

作　　者：夏永华[1] 刘冬[1] 张彩凤[2] 郭金梅[1] 李素娟[1] 付丹丹[1] 李敏[1] 李占国[1] 田中伟[1] 

机构地区：[1]新乡医学院第一附属医院皮肤性病科,河南省卫辉市453100 [2]新乡医学院第一附属医院消化科,河南省卫辉市453100

出　　处：《中华皮肤科杂志》2011年第7期494-496,共3页Chinese Journal of Dermatology

基　　金：河南省高校科技创新人才支持计划（2009HASTIT030）;河南省卫生科技创新型人才工程专项经费（4159）

摘　　要：目的探讨垂体瘤转化基因（mG）小干扰RNA（siRNA）对人皮肤鳞状细胞癌（鳞癌）裸鼠移植瘤生长及浸润和转移相关因子基质金属蛋白酶（MMP）2、9表达的影响。方法应用人皮肤鳞癌细胞株SCL-1细胞建立裸鼠皮下移植瘤模型，观察经FITGsiRNA治疗后移植瘤的生长情况，并利用RT-PCR和Western印迹检测glTGsiRNA治疗后PTrG、MMP-2及MMP-9mRNA和蛋白的表达。结果成功建立人皮肤鳞癌裸鼠皮下移植瘤模型，PTFGsiRNA治疗能明显抑制瘤体的生长（P〈0．05），并下调PTFGmRNA和蛋白的表达。此外，tWFGsiRNA治疗组中其MMP-2和MMP-9的表达均明显下调。结论PTFGsiRNA能抑制人皮肤鳞癌裸鼠移植瘤的生长，并下调MMP-2和MMP-9表达。Objective To investigate the effect of pituitary tumor-transforming gene （PTTG） siRNA on the growth, invasion of, and expression of metastasis-related cytokines including matrix metalloproteinase-2 （MMP-2） and MMP-9 in xenografted human cutaneous squamous cell carcinoma in nude mice. Methods SCL-1 cells were subcutaneously inoculated into Balb/c nude mice to establish a xenograft model of human cutaneous squamous cell carcinoma. Then, 15 mice bearing xenografted carcinoma were equally divided into 3 groups to be inoculated with phosphate buffer saline （PBS）, control siRNA, and P＇ITG siRNA of 50 nmol/L, respectively, every other day for 2 weeks. The size of xenograted carcinoma in these mice was measured every other day. At the end of 2-week treatment, the mice were killed followed by the evaluation of tumor weight, as well as the quantification of mRNA and protein expression of PTTG, MMP-2 and MMP-9 by reverse transcrip- tion （RT）-PCR and Western-blot, respectively. Results The xenograft model of human cutaneous squamous cell carcinoma was successfully established. The treatment with PTTG siRNA obviously inhibited the growth of the xenografted tumors and the expression of trITG mRNA and protein compared with PBS and control siRNA （all P 〈 0.05）. In addition, the expression of MMP-2 and MMP-9 in xenografted tumors in PTTG siRNA- treated mice were significantly lower than those in PBS and control siRNA-treated mice, suggesting that PTYG siRNA evoked the decrease in invasive and metastatic ability of xenografted tumors. Conclusions PTTG siRNA can inhibit the growth of human cutaneous squamous cell carcinoma xenografts in nude mice, and downregulate the expression of invasion- and metastasis-related cytokines, including MMP-2 and MMP-9.

关 键 词：癌基因 癌 鳞状细胞 RNA 小分子干扰 基质金属蛋白酶2 基质金属蛋白酶9 异种移植模型抗肿瘤试验 

分 类 号：R739.5[医药卫生—肿瘤]