Spa-1和Ki-67在乳房外Paget病肿瘤组织中的表达  

Expression of Spa-1 and Ki-67 in tissues of extramammary Paget＇s disease （EMPD）

作　　者：张琛[1,2] 钱悦[1] 冯爱平[1] 陈思远[1] 吴艳[1] 高炳爱[2] 刘岩[2] 徐钧[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉430022 [2]北京航天总医院,100076

出　　处：《中华皮肤科杂志》2011年第7期517-518,共2页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30901288）

摘　　要：目的探讨Spa-1在乳房外Paget病发生和转移中可能的作用。方法取17例原位乳房外Paget病和12例侵袭性乳房外Paget病患者皮损及9例正常人皮肤组织，用免疫组化分别检测Spa-1和Ki-67的表达。结果Spa一1在胞质中显色，Ki-67在胞核中显色。Spa-1和Ki-67仅少量表达于正常人皮肤基底层，在乳房外Paget病肿瘤组织中的表达明显高于正常人皮肤（P〈0．01）。Spa-1在侵袭性乳房外Paget病肿瘤组织中表达明显高于原位乳房外Paget病（P〈0．01）。乳房外Paget病肿瘤组织中Spa-1的表达与Ki-67的表达呈正相关。结论Spa-1在乳房外Paget病的发生发展中可能起作用。Objective To investigate the potential role of Spa-1 in the development and metastasis of EMPD. Methods Tissue specimens were resected from 17 patients with primary EMPD, 12 patients with invasive EMPD and 9 normal human controls. Immunohistochemistry was performed to measure the expression of Spa-1 and Ki-67. Results Positive staining was observed for Spa-1 in cytoplasm, and for Ki-67 in cell nuclei. Spa-1 and Ki-67 were weakly expressed in the basal layer of normal skin. The expression of Spa-1 and Ki-67 in EMPD tissue were statistically higher than those in the normal control tissue （both P 〈 0.01 ）. Increased expression of Spa-1 was noted in invasive EMPD tissue compared with in situ EMPD tissue. The expression of Spa-1 was positively correlated with that of Ki-67 in the tissue of EMPD. Conclusion Spa-1 may play a certain role in the initiation and progression of EMPD.

关 键 词：乳房外PAGET病 KI-67 肿瘤组织 人皮肤组织 免疫组化 侵袭性 SPA 基底层 

分 类 号：R739.5[医药卫生—肿瘤]