中间球海胆放流群体和养殖群体的遗传学比较  

Genetic Analysis of Released and Artificial Cultured Strongylocentrotus intermedius

作　　者：孙博林[1] 张慧敏[1] 王霞[1] 初冠囡[1] 金迪[1] 万良[1] 秦艳杰[1] 

机构地区：[1]大连海洋大学海洋生物资源可持续利用重点实验室,辽宁大连116023

出　　处：《中国农学通报》2011年第17期67-71,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"中间球海胆生长分化现象研究"(30800849);大连市科学技术基金项目"利用分子标记辅助选择法快速建立中间球海胆优良生长家系的研究"(2007J23JH024)

摘　　要：利用微卫星技术,对中间球海胆（Strongylocentrotus intermedius）的放流群体与养殖群体进行了遗传学比较。共用11对微卫星引物,分别对每个群体所有个体进行了扩增分析。每对引物分别扩增出了1~5个等位基因。所有引物在养殖群体中共扩增出36个等位基因,而在放流群体中扩增出了30个等位基因。所得数据经Popgen32软件分析,结果表明,2个群体Nei指数,Shannon氏指数,多态位点比例等遗传学指标没有显著差异,说明研究中所涉及的中间球海胆养殖群体与放流群体之间尚未产生明显的遗传分化。6个等位基因在养殖群体中出现,而在放流群体中缺失。说明养殖群体与放流群体之间仍存在着一定的遗传结构差异。以上研究结果表明,该放流群体尚未达到对中间球海胆进行种质资源保护的最初目的。该研究对中间球海胆养殖、增殖放流状况进行了初步的遗传学评价,为海胆养殖、增殖途径及策略的选择奠定了理论基础。Genetic analyses of released and artificial cultured urchins, Strongylocentrotus intermedius, were conducted with microsatellite technology in this research. Eleven pairs of primers were amplified in all individuals of each urchin population. Results showed that 1 to 5 alleles had been amplified in each of detected loci. In total, 36 alleles were detected in cultured population, while 30 of them in released population. Genetic indicators calculated by Popgen32 software showed that there were no significant differences between these two populations in the Nei＇ s index, the Shannon＇s index and the proportion of polymorphic loci, which indicates that there are no significant genetic differentiations between released and artificial cultured population. There are six alleles were detected in cultured population but not in released ones. Both results indicated that there were still some allele-variations existed between cultured and released urchin population. Results from this research shows that the releasing program is failed to improve germplasm resources of S. intermedius. This research have found the genetic structure of cultured and released urchin populations, which can be a basis of theories and strategies of artificial culture and releasing for enhancement in S. intermedius.

关 键 词：中间球海胆 放流群体 养殖群体 微卫星 遗传学 

分 类 号：Q75[生物学—分子生物学]