脑肿瘤干细胞的增殖活性与微血管密度的相关性研究  

Correlation study of proliferative activity of brain tumor stem cells and microvessel density

作　　者：李明武[1] 牛朝诗[1] 高歌[1] 董永飞[1] 梅加明[1] 李静 傅先明[1] 

机构地区：[1]安徽医科大学附属省立医院神经外科,合肥230001 [2]安徽省脑立体定向神经外科研究所

出　　处：《中国微侵袭神经外科杂志》2011年第5期223-226,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：国家自然科学基金项目(编号:30672166)

摘　　要：目的探讨脑肿瘤干细胞(BTSC)增殖活性与微血管密度的相关性。方法采用免疫组织化学染色检测BTSC标记物CD133、增殖细胞核标记物Ki-67和血管内皮细胞标记物CD31在胶质瘤组织中的表达;采用免疫荧光双染法检测CD133/Ki-67和CD133/CD31的共表达情况;并对BTSC的增殖活性与微血管密度的相关性行统计学分析。结果免疫组化显示:低级别胶质瘤中,CD133、CD31和Ki-67的阳性表达较少;而在高级别胶质瘤中,CD133、CD31和Ki-67阳性表达明显增加。免疫荧光双染显示:随着胶质瘤级别升高,CD133/Ki-67共表达明显增加,且可见CD133/CD31共表达于血管内皮细胞。在胶质瘤组织中,Ki-67、CD31表达均与CD133表达呈正相关(r1=0.758,P1=0.000;r2=0.439,P2=0.000),Ki-67与CD31的表达也呈正相关(r3=0.816,P3=0.000)。结论 BTSC与微血管联系密切,不仅在区域分布上围绕微血管增殖分化,并且在功能上与微血管相互促进和依赖。Objective To research the correlation between the proliferative activity of brain tumor stem cells（BTSC） and microvessel density.Methods The expressions of Ki-67,CD31 and CD133 were detected by immunohistochemistry,while the coexpressions of CD133/CD31 and CD133/Ki-67 were detected by immunofluorescence staining.Then,the correlation between the proliferative activity of BTSC and microvessel density was analyzed by statistical method.Results The positive expression of CD133,CD31 and Ki-67 was lower in low-grade glioma than in high-grade glioma by immunohistochemistry.Immunofluorescence staining showed that the coexpression of CD133/Ki-67 obviously increased with the rise of glioma grade.While CD133/CD31 coexpressed in vascular endothelial cell.In addition,there was a positive correlation between the expression of Ki-67,CD31 and CD133 in gliomas（r1=0.758,P1=0.000;r2=0.439,P2=0.000）,and also a positive correlation between the expressions of Ki-67 and CD31（r3=0.816,P3=0.000）.Conclusions In gliomas,the BTSC and microvessel are closely related to each other not only in the regional distribution but also in biological function.

关 键 词：神经胶质瘤 肿瘤干细胞 微血管密度 

分 类 号：R739.41[医药卫生—肿瘤]