细胞色素C在兔蛛网膜下腔出血后脑血管痉挛中的作用  

Role of Cytochrome-C in cerebral vasospasm after subarachnoid hemorrhage in rabbit

作　　者：游鸿海[1] 王灯亮[1] 康德智[1] 张元隆[1] 

机构地区：[1]福建医科大学第一临床医学院神经外科,福州350005

出　　处：《中国微侵袭神经外科杂志》2011年第5期231-234,共4页Chinese Journal of Minimally Invasive Neurosurgery

基　　金：福建省科技厅重点项目(编号:2009Y0018)

摘　　要：目的观察兔蛛网膜下腔出血(SAH)后基底动脉细胞色素C(Cyt-C)的表达规律,以探讨其在细胞凋亡中的作用及与脑血管痉挛(CVS)的关系。方法 36只新西兰大白兔随机分为对照组(6只)和SAH组(30只)。SAH组采用枕大池二次注血法建立兔CVS模型,对照组行枕大池二次注入生理盐水。应用苏木精-伊红染色观察基底动脉形态学变化,TUNEL法检测基底动脉上细胞凋亡情况,免疫组化检测基底动脉内皮细胞和平滑肌细胞的Cyt-C表达。结果对照组基底动脉结构正常,偶见凋亡细胞和Cyt-C表达。SAH组基底动脉出现相应病理学改变,管腔狭窄呈双相期改变;基底动脉上细胞凋亡和Cyt-C表达均在SAH后增多,于第7天达高峰,第10天逐渐下降。与对照组比较,SAH组内各时间点基底动脉直径和Cyt-C表达差异均有统计学意义(P<0.05)。结论兔CVS模型的基底动脉中存在细胞凋亡,Cyt-C是启动细胞凋亡的重要因素,在CVS发生和发展过程中起重要作用。Objective To observe the expression of Cytochrome-C（Cyt-C） in basilar artery after subarachnoid hemorrhage（SAH） in rabbits,and explore the role of Cyt-C in apoptosis and the relations between the expression of Cyt-C and cerebral vasospasm（CVS）.Methods Thirty-six New Zealand white rabbits were divided randomly into SAH group（n=30） and control group（n=6）.Twice blood injection into the cisterna magna was performed in SAH group and twice normal saline injection into the cisterna magna in control group.The morphological changes of the basilar artery were observed by HE staining,the expression of Cyt-C in the endothelial and smooth muscle cells and apoptosis of the epithelial cells in the basilar artery were detected by immunohistochemical and TUNEL techniques respectively.Results The structure of basilar artery was normal and little expression of Cyt-C or apoptotic cells was observed in control group.While corresponding pathological changes in the structure of the basilar artery occurred in SAH groups,and the lumen narrow showed biphasic patterns.The expressions of Cyt-C and apoptotic cells in the basilar artery increased after SAH,achieved the peak 7 days after SAH and gradually decreased 10 days after SAH.There was statistical significance in difference of the basilar artery diameter and Cyt-C expression at every time point between SAH group and control group（P0.05）.Conclusions Apoptosis exist in the basilar artery of CVS pattern in rabbits,Cyt-C is the important factor for inducing apoptosis,playing an important role in the course of occurrence and development in CVS.

关 键 词：蛛网膜下腔出血 血管痉挛 颅内 细胞色素C类 细胞凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]