bFGF反义寡核苷酸对大鼠自体移植静脉内膜增生的影响  

Effect of bFGF antisense oligonucleotides on intimal hyperplasia of vein autografts of rats

作　　者：田鹏[1] 陈剑秋[2] 孙晋津[2] 王旺河[1] 

机构地区：[1]河南省人民医院微创外科,郑州450003 [2]天津医科大学第二医院普通外科,天津300211

出　　处：《医药论坛杂志》2011年第8期1-2,5,共3页Journal of Medical Forum

基　　金：天津市自然科学基金资助项目(项目编号:013612411)

摘　　要：目的探讨碱性成纤维细胞生长因子(bFGF)反义寡核苷酸对大鼠自体移植静脉内膜增生的影响。方法 Wistar大鼠48只,分为反义寡核苷酸(AODN)组、正义寡核苷酸(SODN)组、随机寡核苷酸(RODN)组、空白对照组。将含300μg寡核苷酸的F127多聚凝胶溶液涂于移植静脉及吻合口外周,对比术后1周、2周移植静脉平均内膜及中膜厚度。并检测血管壁细胞内bFGF蛋白表达。结果术后1周、2周,AODN组内膜厚度、内膜/中膜及bFGF阳性细胞率均小于其它各组,差异均有显著性(P<0.05)。而SODN组、RODN组,空白对照组之间比较,均无显著性差异(P>0.05)。术后2周,SODN组、RODN组、空白对照组内膜增生较术后1周时明显,差异有显著性(P<0.05)。结论 bFGF反义寡核苷酸通过对bFGF基因表达的抑制作用,达到了抑制移植静脉内膜增生的目的 。Objective To discuss the effect of bFGF antisense oligonucleotides on intimal hyperplasia of vein autografts of rats.Methods Totally 48 Wistar rats were selected and were randomly divided into AODN（antisense oligodeoxynucleotide）,SODN（sense oligodeoxynucleotide）,RODN（random oligodeoxynucleotide）,and control groups.The transplanted veins of experimental groups were coated with 300μg oligonucleotide polymer gel（Pluronic F127） just after anastomosis.Vein grafts were got 1 and 2 weeks after operation to measure the mean intimal thickness（IT） and intimal/medial（I/M） thickness ratios,and observe the expression of bFGF protein in intima.Results At 1 week and 2week after operation,the IT and I/M thickness ratios and bFGF positive cell percentages of AODN group were lower than that of the other groups（P0.05）,while there was no significant difference in SODN,RODN and control groups（P0.05）.At 2 weeks,the intimae of vein grafts in SODN,RODN and control groups were thicker significantly as compared with those at 1 week（P0.05）.Conclusions bFGF AODN can reduce vein graft intimal hyperplasia by specifically inhibiting the expression of bFGF gene.

关 键 词：反义 寡核苷酸 碱性成纤维细胞生长因子 静脉移植 内膜增生 

分 类 号：R-332[医药卫生]