铜离子对中国花鲈幼鱼的毒性研究  

Toxicity of Cu^(2+) to Juvenile Perch Lateolabrax maculatus

作　　者：朱友芳 洪万树[2] 林金忠 

机构地区：[1]福建莆田市水产科学研究所,福建莆田351100 [2]厦门大学海洋与环境学院,福建厦门361005

出　　处：《生态毒理学报》2011年第3期331-336,共6页Asian Journal of Ecotoxicology

基　　金：福建省莆田市科技局项目[2009N15(5)]

摘　　要：为给中国花鲈(Lateolabrax maculatus)养殖提供污染生物学的理论数据,研究了中国花鲈幼鱼的铜离子中毒症状和半致死浓度(LC50);检测了幼鱼肝脏的谷胱甘肽(GSH)和丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷丙转氨酶(GPT)和谷草转氨酶(GOT)活力;分析了铜离子胁迫对幼鱼的红细胞总核异率和微核率的影响。结果表明:铜离子对幼鱼24、48、72和96h的LC50分别为1.85、1.66、1.48和0.86mg·L-1。濒死的幼鱼肝脏组织溶解性坏死,鳃部组织出现大量的血窦,幼鱼肝脏的GSH和MDA含量及SOD、GPT和GOT活力对铜离子胁迫反应敏感,而CAT活力则反应不敏感。在铜离子浓度为0.42mg·L-1的浓度组中暴露96h,幼鱼的总核异率和微核率显著高于对照组(p<0.05)。以上结果说明铜离子胁迫损害了中国花鲈幼鱼肝脏的抗氧化系统、肝脏组织和鳃部组织,并具有一定的遗传毒性。In order to provide data of pollution biology for the perch（Lateolabrax maculatus） culture,the toxic symptom and LC50 were studied in the juvenile perch exposed to Cu2＋.The levels of GSH and MDA and activities of SOD,CAT,GOT and GPT were examined;The effects of Cu2＋ stress on the total dyskaryosis and micronuclear rates of the juvenile erythrocytes were analyzed.The results showed that LC50 for juvenile exposed to Cu2＋ for 24,48,72 and 96 h was 1.85,1.66,1.48 and 0.86 mg·L-1,respectively.The liver tissue of agonal individuals necrosed and a mass of blood sinuses occurred in the gill tissue.GSH,MDA,SOD,GPT and GOT in the liver were sensitive,while CAT was not sensitive to Cu2＋ stress.Exposed to Cu2＋ at concentration of 0.42 mg L-1 for 96 h,total dyskaryosis and micronuclear rates of the juvenile erythrocytes in the treated groups were significantly higher（p0.05） than that in the control.Cu2＋ stress not only damaged the liver antioxidant system,liver and gill tissues,but also had definite genetic toxity to the juvenile perch.

关 键 词：中国花鲈 铜离子 半致死浓度 肝脏组织 酶活力 微核测定 

分 类 号：S965.2[农业科学—水产养殖]