幽门螺杆菌对胃癌细胞BGC-823形态及凋亡相关基因表达的影响  

Effect of H.pylori extract on cellular morphology and apoptosis-related gene expression in human gastric cancer BGC-823 cells

作　　者：吴莺[1] 李翔[1] 周红[2] 范钰[3] 张尤历[1] 沈琰[1] 何亚龙[1] 

机构地区：[1]江苏大学附属医院消化科,江苏省镇江市212001 [2]江苏大学基础医学与医学技术学院,江苏省镇江市212013 [3]江苏大学附属人民医院肿瘤科,江苏省镇江市212001

出　　处：《世界华人消化杂志》2011年第17期1767-1772,共6页World Chinese Journal of Digestology

基　　金：江苏省研究生培养创新计划基金资助项目;No.1221270009~~

摘　　要：目的:探讨H.pylori刺激人胃癌细胞BGC-823后,对细胞形态以及凋亡抑制基因Survivin和凋亡基因caspase-3mRNA表达的影响.方法:分别用东亚型H.pylori菌株和西方型H.pylori菌株超声提取物,刺激胃癌细胞BGC-823,显微镜下直接观察细胞形态的变化.同时利用荧光定量聚合酶链反应(QRT-PCR)检测胃癌细胞BGC-823凋亡抑制基因Survivin和凋亡基因caspase-3 mRNA的表达水平.结果:H.pylori刺激胃癌细胞BGC-823后12h,出现蜂鸟表型,呈时间依赖性,24h最明显,东亚型组与西方型组相比,引起的细胞蜂鸟表型百分率明显增加(29.3%±2.1%vs8.0%±2.0%,F=164.73,P<0.05).H.pylori超声提取液刺激胃癌细胞BGC-823后,在东亚型组和西方型组均出现凋亡抑制基因Survivin mRNA的表达量明显上调,与对照组相比caspase-3 mRNA的表达量明显下降(P<0.05);并且东亚型组Survivin mRNA的表达的上调以及cas-pase-3 mRNA的表达较西方型组均明显下降(P<0.05).结论:H.pylori超声提取液可诱导胃癌细胞BGC-823发生形态学改变,并且引起凋亡抑制基因Survivin mRNA表达上调、凋亡基因caspase-3 mRNA表达下降.东西方型H.pylori菌株之间存在生物活性的差异.AIM： To investigate the effect of H.pylori extract on cellular morphology and apoptosisrelated gene expression in human gastric cancer BGC-823 cells.METHODS： After BGC-823 cells were treated with ultrasonic extract of the east Asia type or the Western type of H.pylori strain, the changes in cellular morphology were observed by microscopy, and the expression of survivin and caspase-3 mRNAs was detected by fluorescent quantitative polymerase chain reaction （QRTPCR）. The possible correlation between the expression of survivin mRNA and that of caspase-3 mRNA was also analyzed. RESULTS： Hummingbird phenotype was observed in BGC-823 cells 12 h after stimulation H.pylori extract, which was most obvious at 24 h. The percentage of cells showing hummingbird phenotype was significantly higher in cells treated with the extract of the east Asia type than in those treated with the extract of the Western type （29.3 ± 2.1 vs 8.0 ± 2.0, F = 164.73, P 0.05）. The expression of survivin mRNA was significantly higher and that of caspase-3 mRNA was significantly lower in BGC-823 cells treated with H.pylori extract than in control cells （both P 0.05）. Statistical difference was also noted in the expression of survivin and caspase-3 mRNAs between cells treated with the extract of the east Asia type group and those with the extract of the Western type group. CONCLUSION： H.pylori extract could induce changes in cellular morphology and expression of survivin and caspase-3 mRNAs in human gastric cancer BGC-823 cells. The extract of the east Asia type of H.pylori has more potent biological activity than that of the Western type.

关 键 词：幽门螺杆菌 蜂鸟表型 凋亡相关基因 CagA蛋白分型 

分 类 号：R735.2[医药卫生—肿瘤]