Runx3基因甲基化及Runx3蛋白表达在结肠癌发生中的意义  

Significance of methylation and protein expression of the Runx3 gene in colon carcinogenesis

作　　者：何绍亚[1] 韩盛玺[2] 蒋仁发 向阳生[1] 

机构地区：[1]安岳县人民医院消化内科,四川省资阳市642350 [2]四川省人民医院消化内科,四川省成都市610072

出　　处：《世界华人消化杂志》2011年第17期1860-1863,共4页World Chinese Journal of Digestology

摘　　要：目的:评价Runx3基因启动子区CpG岛甲基化状态及Runx3蛋白表达在结肠癌发生中的作用及临床意义.方法:应用DNA甲基化特异性PCR(MSP)技术检测结肠癌、结肠腺瘤、正常结肠黏膜Runx3基因CpG岛甲基化状态,应用免疫组织化学发法检测Runx3蛋白的表达.结果:MSP方法检测发现Runx3基因CpG岛甲基化在结肠癌组、腺瘤组与正常组之间有显著性差异[23.5%(8/34),20.6%(7/34)vs0.0%(0/0),均P<0.05],而结肠癌组与腺瘤组之间无显著性差异.免疫组织化学检测发现结肠癌组中Runx3蛋白表达阳性率较腺瘤组、正常组之间有显著性差异[17.7%(6/34)vs61.8%(21/34),76.5%(26/34),均P<0.05].结论:Runx3基因启动子区CpG岛甲基化是促进结肠癌发生的重要基因事件,可能与Runx3蛋白失表达有关.AIM： To detect the methylation and protein expression of the Runx3 gene in sporadic colorectal cancer （SCRC）, colonic adenoma, and normal colon tissue and to evaluate their clinical significance in colorectal carcinogenesis. METHODS： Thirty-four colonic cancer specimens, 34 colonic adenoma specimens, and 34 normal colonic tissue specimens were used in this study. The CpG island methylation status of the Runx3 gene was detected by methylationspecific PCR in these specimens, and the protein expression of Runx3 was detected by immunohistochemistry. RESULTS： The rates of methylation of the Runx3 gene in colonic cancer and colonic adenoma were significantly higher than that in normal colonic tissue （23.5%, 20.6% vs 0.0%, both P 0.05）. There was no significant difference in the rate of methylation of the Runx3 gene between colonic adenoma and colonic cancer （P 0.05）. The positive rate of Runx3 protein expression was significantly lower in colonic cancer than in colonic adenoma and normal tissue （17.7% vs 61.8%, 76.5%, both P 0.05）. CONCLUSION： Methylation of the promoter CpG islands of the Runx3 gene is an important genetic event of colon carcinogenesis and may be associated with altered protein expression of Runx3.

关 键 词：RUNX3 甲基化 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤]