分光光度法测定水果中的微量铜  

Determination of trace copper in fruits by spectrophotometry

作　　者：蔡湘雯[1] 张毅忠[1] 李妍[1] 

机构地区：[1]湖南文理学院化学化工学院,湖南常德415000

出　　处：《湖南文理学院学报（自然科学版）》2011年第2期48-50,54,共4页Journal of Hunan University of Arts and Science(Science and Technology)

摘　　要：研究了Cu(Ⅰ)在硫酸和磷酸的混酸介质中与硫氰酸钾、罗丹明B反应生成离子缔合物的显色反应,该显色反应在表面活性剂聚乙烯醇(PVA)的存在下能提高灵敏度,利用该反应在抗坏血酸的作用下测定Cu(Ⅱ),其线性范围为(0.4～10.0)mg/25 mL.该法简便易行,用于测定水果中的微量铜,得到的结果令人满意,能满足水果中微量铜分析的要求.The formation of ion-association complex of Cu（II） with thiocyanida and rhodamine B, an sensitive chromogenic reaction, was investigated in mixed acidic medium consisted of sulphuric acid and phosphoric acid. It was experimentally proven that the chromogenic reaction become more sensitive in the presence of PVA surfactant. Using the chromogenic reaction to determine Cu（II）, linear range was obtained between 0.4 and 10.0 mg/25mL. The method is proved to be simple and successfully applied to the determination of trace Cu（II） in variety of fruits.

关 键 词：分光光度法 罗丹明B 铜 水果 

分 类 号：O657.3[理学—分析化学]