Notch3在卵巢上皮性癌中的表达及临床意义  

Expression of Notch3 in Ovarian Carcinoma and Its Relationship with Angiogenesis

作　　者：王中显[1] 陶涛[1] 周志刚[1] 周婷[1] 卢运萍[1] 王世宣[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《华中科技大学学报（医学版）》2011年第3期264-267,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家重点基础研究发展计划资助项目(No.2009CB521800)

摘　　要：目的探讨卵巢上皮性癌组织中Notch3的表达与卵巢癌血管生成以及临床病理特征的关系。方法应用免疫组织化学SP法检测91例卵巢肿瘤组织中Notch3的表达和CD34标记的微血管密度（MVD）,结合临床病理特征进行综合分析。结果在恶性和交界性卵巢肿瘤组织中Notch3阳性率及MVD计数均显著高于正常卵巢和良性卵巢肿瘤（均P〈0.01）。Notch3在FIGOⅢ-Ⅳ期,低分化和有腹水的卵巢癌患者中的阳性率高于FIGOⅠ-Ⅱ期,高、中分化和无腹水者（均P〈0.05）,MVD计数与患者组织学分级和有无腹水相关（均P〈0.05）,Notch3在卵巢癌组织中的表达与MVD计数之间存在相关性（rs=0.531,P〈0.01）。结论 Notch3可能参与卵巢癌的血管生成,促进卵巢癌的发生发展过程。Objective To investigate the expression of Notch3 and its relationship with angiogenesis in ovarian epithelial carcinoma and its relevance to clinicopathologic features.Methods The expression levels of Notch3 and CD34 were detected by using immunohistochemistry SP method in 91 ovarian tumor specimens.The microvessel density（MVD）was determined with CD34 immunostaining of microvascular endothelial cells.Statistical analysis was performed using the software SPSS（version 12.0）.Results Notch3 positive rate and MVD in borderline malignant ovarian cancer tissues were significantly higher than those in the normal and benign ones（P〈0.01）.Notch3 positive expression rate in patients with ovarian carcinoma with FIGO stage Ⅲ-Ⅳ,poor differentiation and ascites were higher than in those with FIGO stage Ⅰ-Ⅱ and well differentiation and without ascites（P〈0.05）.MVD was related to hitological grade and ascites（P〈0.05）.There was a correlation between Notch3 and MVD（rs=0.531,P〈0.01）.Conclusion The expression of Notch3 in ovarian tumor is related to angiogenesis.Notch3 may be involved in the development and metastasis of ovarian carcinoma.

关 键 词：卵巢肿瘤 NOTCH3 微血管密度 血管生成 

分 类 号：R737.31[医药卫生—肿瘤]