消癌平治疗小鼠恶性腹水的实验研究  

Experimental study of Xiaoaiping on malignant ascites in mice

作　　者：时艳艳[1] 岳麓[1] 齐卫卫[1] 魏红梅[2] 王秀美[1] 邱文生[1] 

机构地区：[1]青岛大学医学院附属医院肿瘤科,山东青岛266003 [2]解放军第八十八医院肿瘤科,山东泰安271000

出　　处：《中华肿瘤防治杂志》2011年第9期655-658,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:观察消癌平注射液治疗小鼠肝癌H22细胞腹水瘤效果,并初步探讨其作用机制。方法:建立小鼠H22腹水瘤模型,随机区组分为生理盐水组和消癌平组,每组小鼠各20只。建模后48 h开始给药,消癌平组连续腹腔给药(0.2 mL/d)6 d,生理盐水组给予同体积生理盐水。末次给药24 h后处死各组小鼠半数。计算各组小鼠平均腹水量,腹水抑制率、腹水中瘤细胞数、肿瘤细胞生存率;并检测腹水肿瘤细胞凋亡、细胞周期及Bcl-2蛋白表达。剩余小鼠继续观察一般状态及生存期。结果:和生理盐水组相比,消癌平组小鼠精神好、动作灵敏、反应快;消癌平组平均腹水体积(7.5±1.3)mL低于生理盐水组平均腹水体积(10.3±1.7)mL,P<0.05;消癌平组腹水中肿瘤细胞数(2.0±1.1)×107明显低于生理盐水组腹水中肿瘤细胞数(6.6±2.8)×107,P<0.01;消癌平组腹水肿瘤细胞生存率(34%)明显低于生理盐水组肿瘤细胞生存率(58%),P<0.001;消癌平组小鼠生存期(16.0±1.5)d高于生理盐水组小鼠生存期(14.2±1.1)d,P<0.05。消癌平组小鼠腹水H22细胞凋亡增加,P<0.05;H22细胞G2/M期百分比增高,P<0.05;Bcl-2蛋白表达减少,P<0.05。结论:消癌平对H22肝癌腹水瘤的生长具有明显抑制作用,其机制可能与下调Bcl-2蛋白表达及诱导细胞凋亡有关。中华肿瘤防治杂志,2011,18(9)OBJECTIVE： To observe the effect of Xiaoaiping in jection on the mice with ascitogenous tumor of hepatic carcinoma H22 and research the potential mechanism. METHODS： A model of ascitic tumor in mice with H22 was created. The mice were random ized to 2 groups： normal saline group and Xiaoaiping group, with 20 mice in each group. Medication started at 48 hours after the models were created. Xiaoaiping （0.2 mL/d） was given through intraperito heal injection for 6 days, the same volume of saline was given to the control group. Half of the mice were killed after 24 hours of the last medication. The average volume of ascites, inhibition rate of ascites, tumor cell number and the cell survival rate were detected. The per centage changes of apoptosis, the cell cycle and the expression of Bcl-2 proteinum of ascitic tumor cells were measured. The living con dition and life span were observed of the other half of the mice. RE SULTS： Compared with normal saline group, mice of Xiaoaiping group were in well condition which were in fine mind, quick action and fast react. The volume of ascites in Xiaoaiping group [（7. 5± 1.3） mL] was lower than that in normal saline group [（10.3± 1.7） mL, P〈0.05]. The number of tumor cells in Xiaoaiping group [（2.04±1. 1）× 10^7] was less than that in normal saline group [（6.6±2. 8） × 10^7 , P〈0. 01]. The survival rate of tumor cells （34%） were decreased in Xiaoaiping group （58%, P〈0. 001）. The life span in Xiaoaiping group [（16.0±1.5） d] was longer than that in the control group [（14.2=]1.1） d, P〈0.05]. In the Xiaoaiping group, the H22 cell apoptosis was increased, the cell cycle was in hibited in G2/M stage and the expression of Bcl-2 proteinum of ascit ic tumor cell was lower than control group （all P〈0.05）. CONCLU SIONS： Xiaoaiping has a significant inhibitory effect on mice bearing H22 ascitic tumor. The mechanism might be related to downregula tion the expression of Bcl-2 and apoptosis of inducer cells.

关 键 词：肝肿瘤/并发症 腹水/治疗 抗肿瘤药 细胞凋亡 

分 类 号：R73-3[医药卫生—肿瘤]