高糖对人牙周膜细胞的生物学作用  

The biological effect of high glucose on human periodontal ligament fibroblast

作　　者：刘加强[1,2] 刘洪臣[1] 王懿[1] 冯元[1] 高辉[3] 

机构地区：[1].解放军总医院口腔研究所,北京100853 [2]解放军第313医院口腔科,辽宁省葫芦岛125000 [3]武警北京总队第三医院口腔科.北京100141

出　　处：《上海口腔医学》2011年第3期225-229,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30973354);教育部博士后科学基金(20100471816)~~

摘　　要：目的:检测高糖环境对体外培养的人牙周膜细胞(hPDLF)增殖、总蛋白合成、碱性磷酸酶活性(ALP)、Ⅰ型胶原(Col-Ⅰ)和骨钙素(OCN)分泌的影响以及胰岛素在此过程中的调节作用,为探讨糖尿病型牙周炎的发病机制及治疗方法提供理论依据。方法:采用含不同糖浓度(5.5、15、25、35、45mmol/L)的培养基培养hPDLF,各组均采用胰岛素作为治疗对照。孵育24h后,采用CCK-8法检测不同糖浓度对细胞增殖的影响;同时检测其对细胞蛋白质合成、ALP活性、Col-Ⅰ和OCN分泌的影响。采用SPSS 13.0软件包对数据进行统计学分析。结果:低浓度的葡萄糖对hPDLF增殖及其他生物学活性无显著影响,随浓度的增高,葡萄糖能够浓度依赖性抑制hPDLF的增殖,减少蛋白质合成及Col-Ⅰ分泌,抑制ALP活性及OCN分泌,这一效应可被胰岛素所抑制。结论:高糖可抑制hPDLF生物学活性,胰岛素能拮抗这种抑制作用。PURPOSE：The investigate the effect of high glucose on proliferation,total protein synthesis,alkaline phosphatase（ALP） activities,collagen-Ⅰ（Col-Ⅰ） and osteocalcin（OCN） secretion of human periodontal ligament fibroblast（hPDLF） in vitro and the regulative function of insulin in this process.METHODS：Glucose of different concentration（5.5,15,25,35and 45mmol/L） was used to culture hPDLF and insulin was used for each group as treatment control.24 hrs after administration,cell proliferation was detected by cell counting kit（CCK-8）,total protein synthesis,ALP activities,Col-Ⅰ and OCN secretion were investigated.Data was analyzed with SPSS 13.0 soft ware package.RESULTS：Glucose of low concentration had no effect on biological function of hPDLF,but glucose of high concentration could inhibit proliferation,total protein synthesis,ALP activities,Col-Ⅰ and OCN secretion of hPDLF significantly in a concentration-dependent manner.This effect could be blocked by insulin.CONCLUSIONS：High glucose could inhibit biological function of hPDLF and such effect could be blocked by insulin.

关 键 词：高糖 hPDLF 胰岛素 细胞生物学活性 

分 类 号：R781.4[医药卫生—口腔医学]