不同浓度氟对大鼠切牙成釉细胞Smad2/3表达的影响  

The effect of different concentrations of fluoride on the expression of Smad2/3 in ameloblast of rat incisor

作　　者：张雪莉[1] 席淑华[2] 郭晓英[2] 程广岩[1] 张颖[1] 

机构地区：[1]中国医科大学口腔医学院口腔预防教研室,辽宁沈阳110002 [2]中国医科大学公共卫生学院地球化学性疾病研究室,辽宁沈阳110001

出　　处：《上海口腔医学》2011年第3期230-233,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30600509);辽宁省自然科学基金(20102278)~~

摘　　要：目的:研究不同浓度氟对大鼠切牙生长过程中成釉细胞内信号转导分子Smad2/3表达的影响,探讨氟斑牙的发病机制。方法:40只Wistar大鼠随机分为3组,建立氟斑牙动物模型。用HE染色和免疫组化方法观察氟对大鼠切牙成釉细胞形态及Smad2/3表达的影响,采用MetaMorph显微图像分析系统和SPSS12.0软件包分别进行图像和数据分析。结果:给氟组大鼠切牙出现典型氟斑牙症状。给氟组Smad2/3的表达显著低于对照组(P<0.01),但低氟组和高氟组间未见显著差异(P>0.05)。结论:过量氟可能通过抑制Smad2/3的表达来影响釉质的分化和发育,导致氟牙症的发生。PURPOSE：To investigate the effect of different concentrations of fluoride on the expression of Smad2/3 which is a specific intracellular signal transduction molecule of TGF-β,and to explore the mechanism of dental fluorosis in rat.METHODS：Forty Wistar rats were randomly divided into 3 groups.HE and immunohistochemistry were used to detect the changes of the ameloblasts and the expression of Smad2/3 in rat incisors.MetaMorph microscope images analysis system and SPSS12.0 software package were used to analyze the images and data.RESULTS：Typical symptoms of dental fluorosis were found in the fluoride group.The expression of Smad2/3 in the ameloblasts in the experimental group was significantly lower than that in the control group（P0.01）;but the difference was not significant between the low-dose group and high-dose group（P0.05）.CONCLUSIONS：By inhibiting the expression of Smad2/3 in ameloblasts,fluoride affects the differentiation and development of enamel,leading to the occurrence of dental fluorosis in rat.

关 键 词：氟化物 大鼠 成釉细胞 SMAD2/3 

分 类 号：R78[医药卫生—口腔医学]