细胞因子诱导的杀伤细胞对食管癌EC9706细胞杀伤作用的实验研究  

Empirical study of cytotoxicity mediated by cytokine-induced killer cells against human esophagus carcinoma cell

作　　者：刘桂举 梅家转[1] 李瑞君[1] 栗敏[1] 林宏伟[1] 

机构地区：[1]郑州人民医院肿瘤内科,河南郑州450003

出　　处：《河南医学研究》2011年第2期134-137,共4页Henan Medical Research

基　　金：郑州市科技计划攻关项目(083SGYS33238-1);郑州市博士创业基金项目(郑卫2009175)

摘　　要：目的:研究细胞因子诱导的杀伤细胞(C IK)对食管癌EC9706细胞的杀伤作用。方法:流式细胞仪检测EC9706细胞NKG2D配体的表达,乳酸脱氢酶释放法测定C IK细胞对EC9706细胞的杀伤活性,裸鼠体内实验验证C IK细胞对肿瘤的影响。结果:EC9706细胞表达M ICA和ULBP2,不表达M ICB,ULBP1和ULBP3;效靶比10∶1、20∶1、30∶1时C IK细胞对肿瘤细胞的杀伤活性分别为(28.81±0.47)%、(37.78±0.22)%和(44.31±1.06)%,提示随着效靶比增高,C IK细胞对肿瘤细胞的杀伤活性明显增强(P<0.05);相关分析表明C IK细胞的杀伤活性与C IK细胞表面NKG2D表达及C IK细胞中CD3+CD56+数目密切相关;体内抑瘤实验对照组和治疗组成瘤时间和瘤重差异均具有统计学意义(P<0.01),治疗组的抑瘤率为35.19%,并且组织病理显示治疗组有较多的淋巴细胞浸润和坏死区。结论:C IK细胞对EC9706细胞具有体内外杀伤作用,有希望成为治疗食管癌的免疫效应细胞。Objective： To investigate the cytotoxicity of cytokine-induced killer （CIK） cells against human esophagus carcinoma cell line（EC9706）.Methods： Expression of NKG2D ligands on EC9706 cells were analyzed by flow cytometry.Cytotoxicity of CIK cells against EC9706 cells was measured using a standard LDH releasing assay.Effect of CIK cells on cancer was tested by nude mice in vivo.Results： It was found that MICA,ULBP2 were expressed,MICB,ULBP1,ULBP3 were not detectable by EC9706 cells.At 10∶1,20∶1 and 30∶1 E∶T ratios,cytotoxicity of CIK cells against cancer cells were respectively（28.81±0.47）%,（37.78±0.22）% and（44.31±1.06）%（P0.05）,and these results indicated that cytotoxicity of CIK cells against cancer cells gradually increased with E：T ratios increasing.Correlation Analysis indicated that the activity of CIK cells against EC9706 cells was closely correlated to the NKG2D and CD3~＋CD56~＋ expressions.At 30：1 E：T ratio,the cytolytic activity of the CIK cells at before and after NKG2D Antibody closure were respectively（44.31±1.06）% and（30.29±1.45）%（P0.05）.The differences in times of the tumor forming and tumor weight between control group and treatment group were statistically significant（P0.01）,and tumor inhibitory rate of treatment group was 35.19%.In addition,histopathology showed that there were more lymphocytes infiltration and necrosis regions in tumor tissue in the treatment group than that in the control group. Conclusion： Our data suggested that CIK cells may be used as a novel adoptive immunotherapy for patients with esophagus carcinoma.

关 键 词：食管癌 细胞因子诱导的杀伤细胞 裸鼠 移植瘤 细胞治疗 

分 类 号：R735.1[医药卫生—肿瘤]