siRNA抑制Ki-67表达对乳腺癌MCF-7/ADR细胞阿霉素耐药性的研究  

Effects of silencing Ki-67 expression by siRNA on the adriamycin resistance of breast cancer cell line MCF-7/ADR

作　　者：李正东[1] 傅韵[1] 成晓林[1] 蒋蓓琪[1] 庄志刚[1] 

机构地区：[1]同济大学附属第一妇婴保健院乳腺外科,上海200040

出　　处：《同济大学学报（医学版）》2011年第3期11-14,19,共5页Journal of Tongji University(Medical Science)

基　　金：上海市卫生局课题(2008y078);上海市科委自然科学基金(092r1424800)

摘　　要：目的采用Ki-67RNA表达载体干扰沉默Ki-67基因,检测其对乳腺癌MCF-7/ADR细胞株Ki-67mRNA及蛋白表达的影响,观察沉默ki-67基因前后乳腺癌细胞株对阿霉素敏感性改变。方法体外合成靶向Ki-67siRNA表达载体,应用脂质体法瞬时转染Ki-67高表达的乳腺癌细胞株MCF-7/ADR,应用RT-PCR和Western印迹法检测转染后Ki-67mRNA及蛋白的表达,MTT法检测细胞增殖活性及转染前后细胞对阿霉素敏感性的变化。结果 Ki-67siRNA载体转染后24 h可显著抑制乳腺癌MCF-7/ADR细胞株的Ki-67mRNA和蛋白表达及细胞的增殖活性。Ki-67siRNA组阿霉素IC_(50)值为(6.3±0.9)μg/ml。结论 Si-Ki-67能够有效抑制Ki-67基因的表达,降低乳腺癌细胞的增殖能力,沉默Ki-7表达能部分逆转阿霉素耐药现象。Objective To investigate the effect of Ki-67 siRNA on doxorubicin resistance of human breast cancer cell line MCF/ADR. Methods siRNA targeting to Ki-67 was synthesized in vitro, then Ki-67 siRNA was transfected to breast cancer MCF-7/ADR cells with liposome. The expressions of Ki-67 mRNA and protein were detected by RT-PCR and Western blotting respectively. The cell proliferation and the measured by MTT method after Ki-67 siRNA transfection. Results Transfection of Ki-67 siRNA significantly inhibited cell proliferation and expression of Ki-67 mRNA and protein expression of MCF-7/ADR cells. The IC50 value of ADM in Ki-67 siRNA-transfected cells was （6.3±0.9）μg/ml. Conclusion Transfection of Ki-67siRNA can inhibit cell proliferation and Ki-67 gene expression in MCF-7/ADR cells, resulting in partial reverse of doxorubicin resistance.

关 键 词：乳腺肿瘤 基因 RNA干扰 化疗耐药 

分 类 号：R737.9[医药卫生—肿瘤]