miR-223在胰腺癌组织中的表达及意义  

The Expression and Significance of MicroRNA-223 in Pancreatic Cancer.

作　　者：王卫卫[1] 刘建强[1] 黄浩杰[1] 邹多武[1] 李兆申[1] 高军[1] 

机构地区：[1]第二军医大学附属长海医院消化内科,上海200433

出　　处：《医学研究杂志》2011年第6期38-41,共4页Journal of Medical Research

基　　金：国家科技支撑计划(2006BAI02A12)

摘　　要：目的分析胰腺癌及其配对癌旁组织中miR-223的表达差异,评价miR-223对胰腺癌潜在的诊断价值。方法收集笔者医院28例胰腺癌患者手术标本的癌组织及配对的癌旁组织,3例正常胰腺组织作为对照。采用TaqMan Real-timePCR方法,以U6为内参,检测miR-223在胰腺癌及配对癌旁组织中的相对表达量,并分析其与胰腺癌临床病理参数的关系。结果胰腺癌组织中miR-223的表达量显著高于癌旁组织(P=0.008),胰腺癌中miR-223的高表达和肿瘤临床特征无关(P>0.05)。结论 miR-223可能在胰腺癌的发生发展中发挥一定作用,并可能成为新的胰腺癌诊断标志物。Objective To detect the expression of miRNA - 223 in pancreatic cancer, paracancerous tissues and normal pancreas tissues and evaluate the diagnositic value of miR - 223 as a diagnostic marker in pancreatic cancer. Methods Twenty - eight cases of pancreatic cancer patients were included in this study. Pancreatic cancer and paraeancerous tissue were collected from every pancreatic cancer patient after surgery. Three normal pancreas were used as control. Relative quantification （RQ） of miR - 223 in tissues were detected by reverse transcriptase PCR and real time PCR. U6 was used as internal control. The relationships between clinicopathologic characteristics of pancreatic cancer and the RQ of miR - 223 were analyzed. Results The RQ of microRNA - 223 in pancreatic cancer tissues were significantly higher than those in paracancerous tissues（P = 0. 008）. The highly expressed miR - 223 did not correlate with clinicopathologic characteristics such as sex, age, tumor maximal diameter and the level of serum CA19 - 9 （P 〉 0.05）. Conclusion The miR - 223 might play an important role in the tumorigenesis and development of pancreatic cancer. It may be used as a new potential diagnostic biomarker for pancreatic cancer.

关 键 词：miR-223 相对表达量 临床特征 

分 类 号：R735.902[医药卫生—肿瘤]