碳纳米管/聚吡咯修饰电极用于液相色谱测定帕金森大鼠脑中神经递质  

Liquid Chromatography with Electrochemical Detection Using Multi-wall Carbon Nanotubes/Polypyrrole Composite Film Modified Electrode for in Vivo Analysis of Monoamine Neurotransmitters in Rat Striatal Microdialysate of Freely Moving Parkinsonian Rats

作　　者：林丽[1,2] 余莉[2] 林瑞泼[2] 李雪燕[2] 杨树林[1] 李校堃[1,2] 

机构地区：[1]南京理工大学化工学院,南京210094 [2]温州医学院药学院化学教研室,温州325035

出　　处：《分析化学》2011年第7期1071-1076,共6页Chinese Journal of Analytical Chemistry

基　　金：浙江省自然基金项目(No.Y2100488);浙江省医药卫生科技计划项目(No.2010QNA015);温州市科技局基金项目(No.Y20090024)资助

摘　　要：制备了碳纳米管/聚吡咯复合修饰电极,研究了多巴胺等单胺类神经递质在该修饰电极上的电化学行为。将此修饰电极作为电化学检测器,与高效液相色谱联用,测定了脑中7种神经递质及其代谢产物。结果表明:去甲肾上腺素、肾上腺素、多巴胺和5-羟色胺的线性范围为5.0"10-10～1.0×10-5 mol/L;3,4-二羟基苯乙酸,5-羟吲哚乙酸和高香草酸的线性范围为1.0×10-9～5.0×10-4 mol/L;7种物质相关系数均大于0.998;检出限在0.1 nmol/L水平。结合微透析活体取样,测定了自由活动帕金森模型组大鼠脑纹状体中7种单胺类神经递质及其代谢产物的含量,较正常组有所降低。A multi-wall carbon nanotubes/polypyrrole modified electrode that can be used as the working electrode in the high performance liquid chromatography with electrochemical detection to determine the monoamine neurotransmitters was developed.The voltammetric response of dopamine could be promoted by using the electrode.The peak currents of norepinephrine,epinephrine,dopamine and 5-hydroxytryptamine were linear with their concentrations ranging from 5.0×10-10 to 1.0×10-5 mol/L,and the peak currents of 3,4-dihydroxyphenylacetic acid,5-hydroxyindoleacetic acid and homovanillic acid were linear with their concentrations ranging from 1.0×10-9 to 5.0×10-4 mol/L.The correlation coefficients of the seven compounds were more than 0.998.The detection limits were the level of 0.1 nmol/L.Coupled with in vivo microdialysis sampling,the method had been successfully applied to measure monoamine neurotransmitters in rat striatum of freely moving Parkinsonian rats,and the monoamine neurotransmitters level of model group decreased compared with control group.

关 键 词：碳纳米管/聚吡咯复合修饰电极 液相色谱电化学检测 微渗析活体取样 神经递质 帕金森病 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R329[医药卫生—临床医学]