木聚糖酶基因xynA在大肠杆菌中的高效表达  

High-level Expression of Xylanase A in Escherichia coli

作　　者：殷尔康[1] 

机构地区：[1]江苏畜牧兽医职业技术学院食品科技系,江苏泰州225300

出　　处：《现代农业科技》2011年第12期326-327,共2页Modern Agricultural Science and Technology

摘　　要：参照Genebank中疏棉状嗜热丝孢菌木聚糖酶A基因序列,以引物拼接方式,人工合成采用大肠杆菌优势密码子的基因xynA,插入pET-20b载体,获得重组表达载体pET-20b-xynA。重组菌在16℃下诱导效果最好,10 h酶活达到42.05 U/mL,重组酶占全细胞蛋白的47%,粗酶在65℃和pH 5～8条件下很稳定。According to the gene sequence in Genebank,a codon-optimized xylanase A gene（xynA）from Thermomyces lanuginosus was syn-thesized to construct the expression vector pET-20b-xynA.16 ℃ was the better induction temperature compared with 37 ℃.A maximum activity of 42.05 U/mL was detected after 10 hours of induction,and the expression level of recombinant xylanase was improved to 47%.The rude enzyme was stable at 65 ℃ and over the pH range of 5.0～8.0.

关 键 词：木聚糖酶A基因 优势密码子 高效表达 

分 类 号：Q78[生物学—分子生物学]