PDE4D、ALOX5AP与缺血性脑卒中的相关性研究  

Study of relationship between the PDE4D,ALOX5AP and ischemic stroke

作　　者：韩晶[1] 梁庆成[1] 吴云[1] 杨春晓[1] 黄爱华[1] 刘寅[1] 

机构地区：[1]哈尔滨医科大学附属第二临床医学院神经内科,黑龙江哈尔滨150086

出　　处：《中国现代医学杂志》2011年第17期1995-1999,共5页China Journal of Modern Medicine

基　　金：省攻关一般项目(No:GC07C35204)

摘　　要：目的探讨磷酸二酯酶4D(PD E4D)单核苷酸多态性(SN P45)、5-脂氧合酶激活蛋白(ALO X 5AP)单核苷酸多态性(SG13S114)基因突变与缺血性脑卒中的关系。方法选取50例急性脑梗死患者为病例组,52例正常对照组,采用聚合酶链反应(PC R)、琼脂糖凝胶电泳技术、限制性内切酶进行酶切以及直接测序分析两组单核苷酸多态性PD E4D基因的SN P45和ALO X 5AP SG 13S114基因,比较两组数据并进行分析。结果 SN P45采用M aeIII GTN AC-限制性内切酶进行酶切后,结果显示对照组中42例能够被切成分子量不同的条带,病例组中38例,病例组和对照组中的大部分人是能被酶切开的,经统计学分析,脑卒中患者SN P45位点的突变率为24%,正常对照组为19%,两组的突变率并没有统计学意义。病例组出现ALOX 5AP基因SG 13S114杂合型(A/T)的(13例,占26%)频率高于正常对照组(11例,占21%),但是两组的差异没有统计学意义(P>0.05)。结论急性脑梗死患者PD E4D基因SN P45基因多态性与缺血性脑卒中的发生无关。急性脑梗死患者ALO X 5AP基因SG 13S114基因多态性与缺血性脑卒中的发生无明显相关性。[Objective] This study is to investigate the association between the mutations in the Single Nucleotide Polymorphisms 45 （SNP45） of PDEdD, SG13Sl14 of ALOXSAP and ischemic stroke. [Methods] In 50 patients with acute stroke and 54 controls, the SNP45 in the PDE4D and SG13Sl14 in the ALOXSAP were analyzed by the PCR, incision enzyme and indirect sequencing. [Results] SNP45 can be enzyme incision by MaeIII^GTNAC, 42 cases in control and 38 cases in IS can be incised two different strap, mutation rate of snp45 is 24% in IS and 19% in control, but it has no significance. There are not exist genetic mutation in the PDFAD and Ischemic Stroke. The frequency of SG13Sl14 in the ALOXSAP in cases （13/50, 26%） was higher than in controls （11/52, 21%）, but it has no significance （P〉0.05）. [Conclusion] The gene of PDE4D is not associated with ischemic stroke. The mutation rate of SG13Sl14 （A/T） in the ALOXSAP of patients with acute stroke is not associated with ischemic stroke.

关 键 词：磷酸二酯酶4D 5-脂氧合酶激活蛋白 缺血性脑卒中 单核苷酸多态性 

分 类 号：R743.33[医药卫生—神经病学与精神病学]