杜鹃花色素的分离与鉴定分析  

Extraction and Primary Identification of Anthocyanidins in Rhododendron Flowers

作　　者：吴丽媛[1,2] 罗向东[1] 戴亮芳[1] 曹娟芳[1] 刘丽萍[1] 洪海燕[1] 潘文艳[1] 

机构地区：[1]江西师范大学生命科学学院,江西南昌330022 [2]四川大学生命科学学院,四川成都610064

出　　处：《食品科学》2011年第13期19-22,共4页Food Science

基　　金：国家自然科学基金项目(30860120;30900781);江西省教育厅科技计划项目(GJJ08159;GJJ09464);江西省自然科学基金项目(2008GQN0059);江西师范大学青年成长基金项目(2398)

摘　　要：运用特征性颜色反应、高效液相色谱(HPLC)和质谱(MS)等技术对浅红色毛叶杜鹃(R.championae Hook)花瓣花色素提取液进行初步鉴定和含量测定。结果表明:该杜鹃品种的花色素含有非红色的黄酮类化合物,主要有黄酮、黄酮醇、二氢黄酮等色素,其中还有部分酚类色素。高效液相色谱(HPLC)显示杜鹃花色素提取液至少包括39种物质,其中有7种可以确定,分别为杨梅黄素3-半乳糖苷、槲皮素3-半乳糖苷、槲皮素-鼠李糖苷、槲皮素3-葡萄糖苷、锦葵素3-阿拉伯糖苷-5葡萄糖苷、杨梅黄素3-鼠李糖苷、绿原酸。利用紫外分光光度法测得杜鹃花瓣中花色苷的含量为131.025mg/100g,总黄酮含量为10.68mg/g。Anthocyanin pigments from Rhododendron championae Hook flowers（light red color） were primarily identified and quantified by the specific color reactions and HPLC-MS/MS.Anthocyanins were extracted by grinding with liquid nitrogen.The resulting extract was found to contain non-red flavonoid compounds（mainly flavonoids,flavonols and flavanones） and some phenolic compounds.HPLC analysis demonstrated that the extract contained at least 39 compounds,of which,7 were identified to be myricetin-3-galactoside,quercetin3-galactoside,quercetin-3-rhamnoside,quercetin-3-glucoside,malva-3-arabinoside-5-glucoside,myricetin-3-rhamnoside and chlorogenic acid,respectively.Rhododendron championae Hook flowers were determined by UV spectrophotometry to contain 131.025 mg/100 g anthocyanin and 10.68 mg/g total flavonoids.

关 键 词：杜鹃 花色素 高效液相色谱-质谱 

分 类 号：S685.21[农业科学—观赏园艺]