黄海北部海域沉积物反硝化细菌数量及反硝化速率的季节变化  

Seasonal variation of denitrifying bacteria and denitrification rate in the sediment of the northern Yellow Sea

作　　者：晨曦[1] 于江华[2,3] 王晓东[2,4] 赵阳国[2] 王纯杰[2] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]中国海洋大学环境科学与工程学院,山东青岛266100 [3]Department of Environm ental Engineering,Hanseo University [4]青岛扬帆船舶制造有限公司,山东青岛266209

出　　处：《海洋科学》2011年第6期48-51,共4页Marine Sciences

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07208-009);国家重点基础研究发展计划(973计划项目2006CB400602)

摘　　要：用乙炔抑制法和最大或然数（most probable number，MPN）法对黄海北部海域沉积物反硝化速率及反硝化细菌数量的季节变化进行了研究，结果表明，该海域反硝化速率在夏季最大，范围在3．2～7．5μmol／（m^2·h）之间，平均值为4．85μmol／（m^2·h）；而在春、秋季其范围分别为0．26～2．65μmol／（m^2·h）和1．21～4．12μmol／（m^2·h）。该研究海域3个季节反硝化细菌数量差别较大，春、夏、秋季分别在1．78×10^4～8．12×10^4，1．18×10^6～6．18×10^6和0．72×10^5～4．50×10^5个／g之间。春、秋两季反硝化速率和反硝化细菌数量之间呈显著性正相关，相关系数分别为0．759和0．750（P〈0．05）。本结果可为黄海北部海域氮循环机制研究提供重要参考。The denitrification rate and denitrifying bacteria in sediment were measured using acetylene inhibition technique and most probable number （MPN） method in different seasons in the northern Yellow Sea. The denitrification rates ranged from 3.2 to 7.5 μmol/（m^2·h）with the average of 4.85 μmol/（m^2·h）. The rates were 0.26 to 2.65 μmol/（m^2·h） and 1.21 to 4.12 μmol/（m^2·h） in spring and autumn, respectively. The denitrifying bacteria were 1.78×10^4～8.12×10^4,1.18×10^6～6.18×10^6 and 0.72×10^5～4.50×10^5 eells/g in spring, summer and autumn, respectively. The result also showed that there were significant positive relationship between denitrification rate and denitrifying bacteria, particularly in spring and autumn. The correlation coefficients were 0.759 and 0.750 （P 〈 0.05） in spring and autumn. The results provided evidences for elucidation of the nitrogen cycling mechanism in the northern Yellow Sea.

关 键 词：反硝化细菌 反硝化速率 季节变化 北黄海 

分 类 号：TS205.7[轻工技术与工程—食品科学]