烟草木质素的Fe-CA仿酶降解  

Biomimetic Degradation of Lignin in Tobacco by Fe-CA System

作　　者：毛耀 刘志昌 姚元军 王凤兰 王磊 谢益民[2] 

机构地区：[1]湖北中烟技术中心重组烟叶应用技术研究湖北省重点实验室 [2]湖北工业大学化学与环境工程学院

出　　处：《烟草科技》2011年第6期48-51,共4页Tobacco Science & Technology

基　　金：国家自然科学基金资助项目"难降解水溶性木素及衍生物的氧化酶催化聚合"(20674046);"木质纤维素与异氰酸酯类的接枝共聚及纸张增强机理的研究"(30871996)

摘　　要：为降低烟草中木质素的含量,提高再造烟叶的质量,制备了Fe-CA仿酶,并通过正交试验优化了烟草(烟梗和碎烟叶片)处理工艺。结果表明:①最优处理条件为:反应温度45℃,处理时间40 min,仿酶用量40 mmol/kg,在此条件下处理的烟草中木质素含量由3.56%降至1.69%,去除率达52.5%;②通过扫描电子显微镜(SEM)观察,处理后的烟草表面变得粗糙不平,有许多孔洞和凹坑,还有纤维骨架露出,表明仿酶可以高效脱除木质素;③仿酶处理后的再造烟叶的香气、杂气和协调性得到了改善,木质杂气减少,刺激性降低,品质得到提升。In order to reduce the lignin content in tobacco and improve the quality of reconstituted tobacco, Fe-CA biomimetic system was prepared and the treatment process of tobacco （stem and scrap） was optimized by orthogonal experiment. The results indicated that the optimal condition was： temperature 45 ℃, treatment time 40 minutes, and application amount of Fe-CA biomimetic system 40 mmol/kg. The lignin content in tobacco decreased from 3.56% to 1.69% , and the removal rate reached 52.5%. SEM observation indicated that, the surface of treated tobacco became rough and scattered with many pores and holes, some fiber framework appeared. It was suggested that Fe-CA biomimetic treatment could reduce lignin in tobacco efficiently. Moreover, the sensory evaluation showed that the Fe-CA biomimetic treatment improved the aroma, harmony and quality of reconstituted tobacco and reduced its offensive odor and irritation.

关 键 词：烟草 再造烟叶 仿酶 木质素 扫描电镜 评吸 

分 类 号：TS411.1[农业科学—烟草工业]