稻瘟病菌分泌蛋白质在致病过程中的作用初探  

Primary Study on Effect of Secreted Proteins of Magnaporthe oryzae During Infection

作　　者：刘永锋[1] 陈志谊[1] 俞文渊[1] 于俊杰[1] 胡建坤[1] 刘邮洲[1] 聂亚锋[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014

出　　处：《中国水稻科学》2011年第4期452-454,共3页Chinese Journal of Rice Science

基　　金：江苏省农业科学院院基金资助项目(人才项目6510803)

摘　　要：鉴定了稻瘟病菌孢子萌发过程中分泌产生的蛋白质。将稻瘟病菌菌株2005-50、2005-81未萌发孢子、萌发孢子以及含有孢子萌发液的萌发孢子分别接种后,病情指数分别为11.13,35.06,49.01和49.6,8.4,62.7;说明孢子萌发液中具有能够帮助稻瘟病菌侵染的物质。利用SDS-PAGE电泳分析稻瘟病菌孢子萌发液,发现孢子萌发液中含有蛋白质类物质。进一步利用生物质谱鉴定和数据库资源检索,共检索到8个稻瘟病菌的蛋白质基因,其基因编号分别为MGG_01305,MGG_13188,MGG_12454等。提示这些蛋白质可能在稻瘟病菌侵染过程中具有重要的功能。Secreted proteins during spore germination of Magnaporthe oryzaewere identified.Ungerminated spores,germinated spores and the mixture of germinated spores with germination liquid of M.oryzae（2005-50,2005-81） were inoculated on rice seedlings,respectively.The disease indices were 11.13,35.06,49.01 and 49.01,8.4,62.7,indicating that germination liquid played a driving role in infection process.SDS-PAGE amplification revealed that secreted proteins were contained.According to the results of mass spectrometer,and database query,eight genes were involved,including MGG_01305,MGG_13188,MGG_12454,etc.The results indicated that these proteins played key roles and important function during M.oryzae infection.

关 键 词：稻瘟病菌 分泌蛋白 孢子萌发 质谱 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]