不同载体对癌胚抗原DNA疫苗诱导小鼠产生抗CEA水平的影响  

Effects of different vectored CEA DNA vaccines on anti CEA production

作　　者：张光磊[1] 张彤[1] 陈宗德[1] 张燕齐[1] 阮丽荣[1] 张绍祖[1] 

机构地区：[1]河南医科大学微生物学与免疫学教研室,郑州450052

出　　处：《中华微生物学和免疫学杂志》1999年第6期516-518,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的　将癌胚抗原（ＣＥＡ） 基因克隆入载体ｐｃＤＮＡ３ 及ＶＲ１０１２ 中，比较两套重组质粒在体内表达并诱导小鼠产生抗ＣＥＡ 的差别。方法　通过基因工程技术构建两套ＣＥＡ 真核表达载体ｐｃＤＮＡ３ＣＥＡ 及ＶＲ１０１２ＣＥＡ，将重组质粒肌注ＢＡＬＢ／ｃ 小鼠，运用ＥＬＩＳＡ 法检测不同时间肌肉组织表达ＣＥＡ 水平及血清中抗ＣＥＡ 的产生。结果　ｐｃＤＮＡ３ＣＥＡ 在肌肉内呈低表达ＣＥＡ，最高时为０－１８ｎｇ／ ｍｇ 肌肉（ 胫前肌） ，而ＶＲ１０１２ＣＥＡ 表达量为０－９３ｎｇ／ ｍｇ 肌肉，但两重组质粒诱导小鼠产生的抗ＣＥＡ 的水平相差不大，均大于１ ２００ 。结论　ＤＮＡ 疫苗诱导的免疫反应不仅取决于其在体内的表达抗原量，质粒结构中的免疫激活序列（ＩＳＳ） 对免疫反应也起重要的调节作用。Objective To study the anti CEA induced respectively by pcDNA3 CEA and VR1012 CEA DNA vaccines. Methods Genetic engineering techniques were applied to insert a 2.4kb fragment encoding full length human carcino embryonic antigen (CEA) respectively into two eukaryotic expression vectors, pcDNA3 (containing immunostimulatory sequences, ISS), and VR1012. Recombinant plasmids were injected into tibias anterior of BALB/c mice. ELISA was used to examine local expression of CEA. Cell ELISA was applied to the detection of anti CEA production. Results pcDNA3 CEA expressed low level of CEA in muscles, only 0.18ng/mg muscle on day 7 post injection, whereas VR1012 CEA yielded 0.93ng/mg of CEA in situ. Despite its low level expression, pcDNA3 CEA induced higher titer of anti CEA over 1200 , which was just hardly less than that induced by VR1012 CEA. Conclusion The type and intensity of immune responses elicited by DNA are thought not only to depend on the amount of in vivo expression of antigens, but also some key motifs in the plasmid backbone of DNA vaccines, which seem to play important roles in modulating immune responses. The research has laid a foundation for designing more effective vectors for DNA vaccination purposes.

关 键 词：癌胚抗原 DNA疫苗 免疫反应 

分 类 号：R730.3[医药卫生—肿瘤]