高温气相色谱分析甘油酯的研究  

Analysis of acylglycerols by high temperature gas chromatography

作　　者：王丽丽[1] 汪勇[1] 胡长鹰[1] 张云[2] 

机构地区：[1]暨南大学食品科学与工程系,广州510632 [2]安捷伦科技有限公司,上海200131

出　　处：《中国油脂》2011年第7期75-79,共5页China Oils and Fats

基　　金：国家"863"计划项目(2010AA101505);国家自然科学基金项目(31000793);粤港关键领域重点突破项目(2009A020700003);广东省科技计划项目(2G09B080701063)

摘　　要：建立了高温气相色谱分析油脂样品中脂肪酸和甘油酯含量的方法。采用保留时间定性,外标法定量,定性定量准确。得到的脂肪酸、单甘酯、甘油二酯、甘油三酯的标准曲线,在质量浓度范围0.5～20.0 mg/mL内峰面积与被测物质量浓度线性关系良好。加样回收试验表明,平均加样回收率在95.69%～105.78%之间,RSD在0.27%～2.98%之间。用该方法分析各种脂肪酸和甘油酯均有较好的精密度和重复性。A high temperature gas chromatography method was developed for analyzing the content of fatty acid （FFA） and acylglycerols in oils samples. FFA and aeylglycerols were identified by the retention time with standards and quantified by external standard method, which was turned out to be correct. The calibration curves of FFA, monoacylglyeerol （ MAG）, diaeylglycerol （DAG） and triacylglycerol （TAG） built by the high temperature gas chromatography showed a good linear correlation at concentration range of O. 5 -20.0 mg/mL. Recovery experiments showed the average recoveries of FFA and acylglycerols were at range of 95.69% - 105.78% with RSD at range of 0.27% -2.98%. The method had good precision and reproducibility.

关 键 词：高温气相色谱 甘油酯 毛细管柱 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]