一株多菌灵高效降解菌的分离鉴定及特性  

A high efficient carbendazim-degrading bacterial strain:Its isolation and identification

作　　者：林秀[1] 侯振安[1] 赵思峰[1] 谢磊[1] 李会杰[1] 李谟志[1] 

机构地区：[1]石河子大学农学院资源与环境科学系,新疆生产建设兵团绿洲生态农业重点实验室,新疆石河子832003

出　　处：《生态学杂志》2011年第7期1479-1483,共5页Chinese Journal of Ecology

基　　金：国家科技支撑计划课题(2007BAC20B04)资助

摘　　要：从长期施用多菌灵农药的葡萄园中分离筛选得到一株多菌灵降解菌XJ-H,经Biolog微生物自动分析系统和16S rDNA序列比对以及系统聚类分析等鉴定菌株XJ-H为巴西固氮螺菌(Azospirillum brasilense)。多菌灵降解菌XJ-H可利用多菌灵为唯一碳源、氮源进行生长,将其接种在600 mg.L-1多菌灵的无机盐培养基中,11 d时多菌灵的降解率达到95.6%,平均降解能力为52.2 mg.L-1.d-1。By the method of continuous enrichment culture, a high efficient carbendazim-degrad- ing bacterial strain XJ-H was isolated from the grape vineyard soil with a long-term application of carbendazim in Shihezi of Xinjiang, Northwest China. Through the Biolog analysis, 16S rDNA sequences comparison, and phylogenetic analysis, the XJ-H was identified as AzospiriUum brasilense. The XJ-H could utilize carbendazim as the sole carbon or nitrogen source. After inoculated into the mineral salt medium containing 600 mg · L^-1 of carbendazim, the XJ-H could degrade 95.6% of carbendazim within 11 d, with an average degradation rate of 52. 24 mg ·L^-1· d^-1.

关 键 词：多菌灵 AZOSPIRILLUM brasilense 分离鉴定 

分 类 号：X172[环境科学与工程—环境科学]