[BMIM]PF_6/缓冲液两相体系中固定化β-葡萄糖醛酸苷酶催化特性研究  

Experiment Study of Catalysis Features of Immobilized β-Glucuronidase in Ionic Liquid PF_6/Buffer Biphasic System

作　　者：曹红[1] 孙倩[2] 段海燕[1] 叶海[1] 陈思羽[1] 刘桂艳[3] 

机构地区：[1]石河子大学化学化工学院,新疆石河子832000 [2]石河子大学食品学院,新疆石河子832000 [3]北京理工大学生命学院,北京100081

出　　处：《北京理工大学学报》2011年第6期732-736,共5页Transactions of Beijing Institute of Technology

基　　金：国家自然科学基金资助项目(20776017;20976014);国家教育部科学技术研究重点资助项目(209146);新疆生产建设兵团博士资金资助项目(2009JC13)

摘　　要：利用1-丁基-3-甲基咪唑六氟磷酸盐([BMIM]PF6)/缓冲液两相体系为介质,固定化β-葡萄糖醛酸苷酶催化甘草酸(GL)合成单葡萄糖醛酸甘草次酸(GAMG),以期改善酶催化效能.实验表明,当反应条件固定化酶为0.25g,两相体系中[BMIM]PF6的体积分数为35%,pH为5.0,反应温度为40℃时,酶活力最高达1 000U,远大于纯缓冲液单相体系中的最高酶活力450U.离子液体重复利用实验表明,[BMIM]PF6回收率高于85%,在含有重复回收的[BMIM]PF6介质中,固定化酶相对活力大于93%.With the purpose of improving enzyme catalytic efficiency, the ionic liquid/buffer biphasic system was used as bio catalytic medium for immobilized β-glucuronidase to catalyze hydrolysis of GL into GAMG. Experiments show that, under the conditions of 0.25 g amount of immobilized enzyme, 35% volume fraction of [BMIM]PF6, pH value of 5.0 and the reaction temperature of 40 ℃, the maximum enzyme activity can reach 1 000 U. It is well above 450 U, which was the highest enzyme activity measured in the buffer mono-phase system. The reutilizing experimental results show that the reusing rate of [BMIM]PF6 is greater than 85%, and the immobilized β-glucuronidase relative activity is above 93% in the reaction medium of recycling [BMIM]PF6.

关 键 词：离子液体 [BMIM]PF6 固定化β-葡萄糖醛酸苷酶 催化特性 

分 类 号：Q556[生物学—生物化学]