RNA干扰对胆囊癌细胞人端粒酶逆转录酶基因表达的影响  

Experimental study on the inhibitory effect of RNA interference on the expression of hTERT mRNA and protein in human gallbladder cancer cell lines

作　　者：吴斌全[1] 邹敏 刘会春[1] 鲁正[1] 

机构地区：[1]蚌埠医学院第一附属医院肝胆外科,安徽省蚌埠市233004 [2]淮南东方医院集团潘三医院检验科,安徽省淮南市232096

出　　处：《实用医学杂志》2011年第13期2315-2318,共4页The Journal of Practical Medicine

基　　金：安徽省高校优秀青年人才基金项目(编号:2009SQRZ133)

摘　　要：目的:观察RNA干扰(RNA interference)对人胆囊癌GBC-SD细胞株中人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)表达的抑制作用。方法:设计靶向hTERT基因的微小RNA(miRNA),构建4个重组表达质粒并转染人胆囊癌GBC-SD细胞株。48h后用RT-PCR和Western blot方法检测hTERT的mRNA和蛋白表达情况。结果:与对照组相比,靶向hTERT的4个重组质粒转染组的hTERT mRNA水平和蛋白表达量均明显降低(P<0.01)。结论:靶向hTERT基因的特异性miRNA表达载体能有效地下调GBC-SD细胞中hTERT mRNA及蛋白表达水平。Objective To evaluate the inhibitory effect of RNA interference on the expression of human telomerase reverse transcriptase （hTERT） mRNA and protein in human gallbladder cancer GBC-SD cell lines. Methods After miRNA targeting hTERT gene was designed, 4 recombinant plasmid vectors were constructed and transfeeted into human gallbladder cancer GBC-SD cells. After 48 hours, mRNA and protein levels of hTERT were determined by RT-PCR and western blot techniques, respectively. Results As compared with control group, the expressions of hTERT mRNA and protein were both significantly decreased after treated by 4 recombinant plasmids hTERT-targeted miRNA-1,-2,-3 and-4 （P 〈 0.01 ）. Conclusion The miRNA expression vector targeting hTERT gene could effectively down-regulate the expression of hTERT mRNA and protein in GBC-SD cells.

关 键 词：胆囊肿瘤 RNA干扰 微小RNA 人端粒酶逆转录酶 

分 类 号：R735.8[医药卫生—肿瘤]