昆虫病原线虫共生菌对小麦根腐病菌和小麦赤霉病菌的抑菌活性  

Antibiotic Activities of the Entomopathogenic Nematodes Symbiotic Bacteria to Bipolaris sorokinianum and Fusarium graminearum

作　　者：王欢[1,2] 刘彦群[2] 董辉[3] 李天来[1] 丛斌[3] 

机构地区：[1]沈阳农业大学园艺学院,辽宁沈阳110866 [2]沈阳农业大学生物科学技术学院.辽宁沈阳110866 [3]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《麦类作物学报》2011年第3期549-553,共5页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30800803);农业部公益性行业专项(200803001);辽宁省自然科学基金项目(20022084);沈阳农业大学青年教师科研基金项目(2005002)

摘　　要：为探索小麦根腐病和小麦赤霉病可持续控制的有效生防途径,以小麦根腐病菌和小麦赤霉病菌作为供试真菌,评价了昆虫病原线虫共生菌及其毒素的抑菌活性,并对高毒力菌株抗逆性进行了初探。结果表明,供试的28个昆虫病原线虫共生菌发酵液和毒素均有一定的抑菌活性,其中,高毒力共生菌菌株SY5发酵液和毒素对小麦根腐病菌的抑菌率分别为56.05%和67.41%,对小麦赤霉病菌的抑菌率分别为82.41%和83.32%;菌株SY5发酵液经50℃处理60 min及18 W紫外灯照射120 min后对小麦根腐病菌的抑菌率无明显变化,但对小麦赤霉病菌的抑菌率有所下降;常温保存150 d,抑菌活性略有下降。说明共生菌菌株SY5对小麦根腐病菌和小麦赤霉病菌抑菌活性显著,且具有一定的抗逆性,极具应用潜力。To provide a method for sustainable control of Bipolaris sorokinianum and Fusarium graminearum.the antibiotic activities of entomopathogenic nematodes symbiotic bacteria on them were investigated through a large number of bioassays.Bioassay results showed that the broth and crude extract of SY5 had high antibiotic activity to Bipolaris sorokinianum and Fusarium graminearum,the inhibiting rate were 56.05%,67.41% and 82.41%,83.32%,respectively.The antibiotic activity of highly virulent strain SY5 broth to Bipolaris sorokinianum was substantially stable to heat at 50℃ for 60 min and to ultraviolet ray for 120 min,but the the antibiotic activity to Fusarium graminearum decreased.The antibiotic activity of the SY5 broth decreased at room temperature for 150 days.It was suggested that the SY5 broth had great potential for controlling Bipolaris sorokinianum and Fusarium graminearum.

关 键 词：昆虫病原线虫共生菌 小麦根腐病菌 小麦赤霉病菌 抑菌活性 

分 类 号：S512.1[农业科学—作物学] S432.1