通络救脑注射液对大鼠脑缺血再灌注损伤脑保护的IGF-1途径分析  

Effects of Tongluo-Jiunao Injection on Cerebral Ischemia-Reperfusion Injury Rats

作　　者：汤轶波[1] 唐卉凌[1] 张玮[1] 王润俊[1] 闫杨杨[1] 徐丽伟[1] 李澎涛[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《世界科学技术-中医药现代化》2011年第3期488-492,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部“重大新药创制”科技重大专项(2009ZX09502-014):基于脑病治疗的中药复方药理学及药效学评价关键技术研究,负责人:李澎涛;国家重点基础的研究发展计划(“973”计划)(2005CB52331):络病学说与针灸理论防治的基础研究通络方药对脑缺血损伤的脑保护作用的微血管机制研究,负责人:李澎涛

摘　　要：目的:观察通络救脑注射液对大鼠局灶性脑缺血再灌注损伤的保护作用及探讨其发挥保护作用的可能途径。方法:以线栓法构建大鼠大脑中动脉局灶缺血再灌注损伤模型,实验动物随机分为假手术组、模型组、通络救脑药物组、阳性药对照(依达拉奉)组。各设3个时间点,分别为脑缺血再灌注后24h、3d和7d,以TTC染色法测定脑组织梗死体积;以酶联免疫(ELISA)、免疫组化法分别检测血清、脑组织匀浆IGF-1表达水平及表达部位。结果:通络救脑注射液能显著减小各时间点的大鼠脑梗死体积比;缺血再灌注3d通络救脑组大鼠血清IGF-1水平显著升高,模型组和通络救脑组大鼠脑匀浆IGF-1水平与假手术组比较显著提高;免疫组化亦提示缺血侧皮层IGF-1的阳性表达面积比在通络救脑组均显著高于相应时间点的模型组。结论:通络救脑注射液能显著减小局灶性脑缺血再灌注大鼠的脑梗死体积,且与提高IGF-1的表达有关。This study aimed to investigate the protective effects of Tongluo-Jiunao injection （TLJN） on brain tissues of fo- cal cerebral ischemia-reperfusion injury rats. Cerebral ischemia-reperfusion injury rat model was established by middle cerebral artery occlusion. All animal models were divided into four groups, which were sham, model, TLJN, and edaravone group. The brain infarct volume, concentration of IGF-1 in brain tissues and serum were detected at 24 h, 3 d, and 7 d after cerebral ischemia-reperfusion injury. The results showed that compared to the model group, the brain infarct volume of TLJN group was significantly reduced, and the expression of IGF- 1 of cerebral cortex was upregulated in TLJN group. It is concluded that TLJN has significant protective effects on brain tissues in focal cerebral ischemia-reperfusion injury rat. And the IGF- 1 may play an important role in the protective effects of brain tissues.

关 键 词：通络救脑注射液IGF-1神经元 

分 类 号：R743.31[医药卫生—神经病学与精神病学]