子宫内膜腺癌中孕激素依赖的cyclin G1与PRA/PRB表达的相关性及临床意义  

Correlation between Progesterone Dependent Cyclin Gl and PRA/PRB Expression in Endometrial Adenocarcinoma and the Related Clinical Significance

作　　者：方明[1,2] 于海礼[1] 袁东智[1] 徐倩[1] 张金虎[1] 何亚平[1] 岳利民[1] 

机构地区：[1]四川大学基础医学与法医学院生理教研室,成都610041 [2]成都大学医护学院生理教研室,成都610106

出　　处：《肿瘤预防与治疗》2011年第4期137-141,共5页Journal of Cancer Control And Treatment

摘　　要：目的:探讨孕激素依赖的cyclin G1与孕激素受体亚型PRA和PRB在子宫内膜腺癌中表达的相关性及cyclin G1在子宫内膜腺癌中低表达的原因。方法:采用免疫组化SP法分别检测48例子宫内膜腺癌组织标本(高分化腺癌17例,中分化腺癌19例,低分化腺癌12例)中cyclin G1与PRA、PRB蛋白的表达,显微镜下计数阳性细胞百分数,以5%作为判断标本阳性表达的标准,同时用BI 2000图像分析系统测定灰度值反映蛋白表达水平,分析在不同分化程度的子宫内膜腺癌中PRA、PRB和cyclin G1表达的差异及其与子宫内膜腺癌病理分级的关系,同时分析cyclinGl表达与PRA、PRB的相关性。结果:48例高、中、低分化子宫内膜腺癌中,PRA阳性表达分别为88.2%(15/17)、79.0%(15/19)、66.7%(8/12),PRA的表达与病理分级无关联性(P>0.05);PRB阳性表达分别为47.1%(8/17)、15.8%(3/19)、8.3%(1/12),cyclinGl阳性表达分别为35.3%(6/17)、10.5%(2/19)、0%(0/12),PRB和cyclin G1的表达与病理分级有关联性(P<0.05),即随分化程度降低而明显降低;cyclin G1表达与PRB表达有关联性(P<0.05),与PRA的表达无关联性。结论:子宫内膜腺癌中PRB和cyclin C1的表达具有关联性,都随肿瘤分化程度降低而降低。子宫内膜腺癌中PRB低表达可能是导致cyclin C1低表达的原因之一。Objective： To study the correlation between cychn G1 and progesterone receptor isoforms PRA / PRB in endometrial adenocarcinoma in order to explore the reason for the low expression of cyclin G1 in this kind of disease. Methods： Immunohistochemistry was used to test the expression of cyclin G1 and progesterone receptor PRA / PRB in 48 cases of adenocarcinoma of endometrium including 17 cases of well-differentiated carcinoma, 19 cases of mid-differentiated carcinoma and 12 cases of poor-differentiated carcinoma. The correlation between cyclin G1 protein expression and PRA or PRB expression was analyzed. Results： In well, mid and poor differentiated adenocarcinoma of endometrium, PRA positive rates were 88.2% （ 15/17 ）, 79.0% （15/19） and 66.7% （8/12） respectively. Statistical analysis showed that there was no correlation between PRA expression and histological grade of endometrial adenocarcinoma（ P 〉 0.05） ; PRB positive rates were 47.1% （8/17）, 15.8% （3/19） and 8.3% （1/12） respectively and eyclin G1 positive rates were 35.3% （6/17）, 10.5% （2/19） and 0% （0/12）respectively. The expression of both PRB and cyclin G1 werepositively correlated with histological grade of endometrial adenocarcinoma（ P 〈 0. 05 ）. There was no correlation between cyclin G1 expression and PRA while relationship was found between cyclin G1 expression and PRB （P 〈 0.05 ）. Conclusion： In endometrial adenocareinoma, the expressions of PRB and cyclin G1 protein decrease with the descending of tumor histological grade, and the expressions of both proteins are positively correlated. Thus it can be deduced that low expression of progesterone dependent cyclin G1 may be related to low expression of PRB in endometrial adenocarcinoma.

关 键 词：子宫内膜腺癌 cyclin G1 PRA PRB 相关性 

分 类 号：R737.33[医药卫生—肿瘤]