尿纤溶酶原激活物及血管内皮生长因子在食管癌中的表达及对肿瘤血管形成的影响  

Expression of urokinase-type plasminogen activator （uPA） and vascular endothelial growth factor （VEGF） in esophageal cancer and their influence to tumor angiogenesis

作　　者：姜建涛[1] 周斌[1] 张淑群[2] 李少民[1] 张潍[1] 张晋[1] 乔哲[1] 孔冉冉[1] 马跃峰[1] 

机构地区：[1]西安交通大学医学院第二附属医院胸外科,710004 [2]西安交通大学医学院第二附属医院肿瘤科,710004

出　　处：《肿瘤研究与临床》2011年第6期372-375,共4页Cancer Research and Clinic

基　　金：陕西省科学技术研究发展计划项目（2009K12-01）

摘　　要：目的探讨尿纤溶酶原激活物（uPA）、血管内皮生长因子（VEGF）在食管癌中的表达及对肿瘤血管生成的影响。方法采用免疫组织化学sP法检测正常食管黏膜上皮组织（18例）及食管癌组织（68例）中uPA、VEGF的表达，检测CD。用以标记肿瘤微血管密度（MVD），根据MVD均值分为高、低MVD组，分析食管癌uPA、VEGF的表达和临床病理特征的关系及对肿瘤血管形成的影响。结果uPA蛋白在正常食管黏膜上皮组织、食管癌组织中的阳性率分别为27．8％（5／18）和70．6％（48／68），差异有统计学意义（X^2=11．63，P〈0．05）；VEGF蛋白在正常食管黏膜上皮组织、食管癌组织中的阳性率分别为22．2％（4／18）和63．2％（43／68），差异有统计学意义（X^2=9．78，P〈0．05）。食管癌组织中uPA与VEGF表达有一致性（X^2=9．72，P〈0．05）。MVD平均为42．38±11．62，高MVD组uPA、VEGF蛋白表达显著高于低MVD组（X^2值分别为6．13和10．12，均P〈0．05）。uPA、VEGF蛋白表达与年龄、性别、病理类型无关（均P〉0．05），均与临床病理分期、分化程度和淋巴结转移相关（P〈0．05）。结论食管癌组织中uPA、VEGF蛋白高表达，可能促进肿瘤血管形成，提示预后不良。Objective To investigate the expression and influence to tumor angiogenesis of urokinase-type plasminogen activator （uPA） and vascular endothelial growth factor （VEGF） in esophageal carcinoma. Methods The expression of uPA and VEGF in the tissue of normal （18 cases） and esophageal carcinoma （68 cases） were evaluated by SP immunohistoehemistry, CD34 was detected as marking tumor microvessel density （MVD）. uPA and VEGF expression were assessed as to the pathologically biological features of esophageal cancer and to the influence to tumor angiogenesis. Results The positive rates of uPA were 27.8 % （5/18） and 70.6 % （48/68） in the tissue of normal and esophageal carcinoma, respectively, there was significant difference in two tissues （X^2 =11.63, P 〈0.05）. The positive rates of VEGF were 22.2 % （4/18） and 63.2 % （43/68） in the tissue of normal and esophageal carcinoma, respectively, there was significant difference in two eissues （X^2=9.78, P 〈0.05）. The expressions of uPA and VEGF in esophageal carcinoma were uniformity （X^2=9.72, P 〈0.05）. The mean of MVD was 42.38＋11.62. The positive rates of uPA and VEGF were higher in the high MVD group than those in the low MVD group （X^2=6.13, P 〈0.05, X^2=10.12, P 〈0.05, respectively）, uPA and VEGF expressions in malignant tumors weren＂ t associated with age, gender and pathological types （P 〉0.05）, but associated with clinical stage, histologic grading and lymph node metastasis （P 〈0.05）. Conclusion Rising expression levels of uPA and VEGF are common in esophageal carcinoma. Altered expression of uPA and VEGF ,nay contribute to tumor angiogenesis of esophageal carcinoma, whose overexpression indicate worse prognosis.

关 键 词：尿纤溶酶原激活物 血管内皮生长因子类 食管肿瘤 新生血管化 病理性 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]