人类X-盒结合蛋白1在肝癌中的表达及其临床病理意义  

Expression and clinical significance of X-box binding protein 1 in liver cancer

作　　者：吕海涛[1] 刘三光[1] 张建生[1] 王文斌[1] 刘润田[1] 闫长青[1] 边伟[1] 马书贵 

机构地区：[1]河北医科大学第二医院肝胆外科,石家庄050000 [2]河北省清河县人民医院外科,石家庄050000

出　　处：《肿瘤研究与临床》2011年第6期390-392,共3页Cancer Research and Clinic

摘　　要：目的研究人类X-盒结合蛋白1（XBP1）在mRNA水平上的表达及其在肝癌发生、发展中的可能作用。方法采用反转录聚合酶链反应（RT—PCR）方法检测了XBPl mRNA在42例肝癌、15例癌旁肝组织和15例正常肝组织中的表达情况，与内参照GAPDHmRNA比较计算相对表达量，分析XBP1表达与肝癌临床病理特征的关系。结果XBP1 mRNA在肝癌组织中的相对表达量（0．4396±0．0241）高于癌旁肝组织以及正常肝组织（分别为0．4152±0．0252和0．4095±0．0149），差异有统计学意义（F=10．94，P=0．001）。肝癌组织中XBPlmRNA的表达与患者年龄、性别、肿瘤部位、组织学类型、分级及有无转移无明显相关性（均P〉0．05）。结论肝癌组织中XBPlmRNA表达明显升高，但其表达与肝癌临床病理特征无关，提示XBP1可能参与肝癌发生，是肝癌发生的早期事件。Objective To investigate the possible role of X-box binding protein （XBP1） expression at mRNA level in the hepatocarcinogenesis. Methods The expression of XBP1 at mRNA level was determined with RT-PCR method in liver carcinoma （42 cases）, liver tissue adjacent to carcinoma （15 cases） and normal liver tissue （15 cases）. XBP1 mRNA relative expression level was obtained by comparing with GAPDH mRNA level. The correlation between XBP1 expression and clinicopathological features of liver carcinoma were respectively analyzed. Results RT-PCR results showed that the expression of XBP1 mRNA in liver carcinoma cases （0.4396±0.0241） was significantly higher that that of liver tissues adjacent to carcinoma （0.4152±0.0252） and normal liver tissue （0.4095±0.0149）, （F=12.79,P =0.001）. No significant differences in the expression of XBP1 were found among different age and sex of the patients, different location, histological types, grade and metastatic status in liver carcinoma （P 〉0.05）. Conclusion Increased expression of XBP1 mRNA is found in liver carcinoma but no correlation could be seen between XBP1 expression and clinicopathological features, which XBPI is involved in the hepatocarcinogenesis.

关 键 词：肝肿瘤 反转录聚合酶链反应 X-盒结合蛋白 

分 类 号：R735.7[医药卫生—肿瘤]