食管鳞状细胞癌组织中HIF-1α和AQP1蛋白的表达  

Expression of hypoxia-inducible factor-1α and aquaporin 1 in esophageal squamous cell carcinoma tissue

作　　者：张红[1,2] 李真珍 王志永[2,3] 赵阿红[1] 高冬玲[1] 

机构地区：[1]郑州大学第一附属医院病理科,郑州450052 [2]河南省高校临床医学重点学科开放实验室,郑州450052 [3]郑州市第一人民医院病理科,郑州450052

出　　处：《郑州大学学报（医学版）》2011年第3期335-338,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技厅普通科技攻关基金资助项目092300410069;河南省卫生厅创新人才基金资助项目200703046

摘　　要：目的:检测食管鳞状细胞癌(ESCC)组织中缺氧诱导因子-1α(HIF-1α)和水通道蛋白1(AQP1)蛋白的表达。方法:应用免疫组化SP法检测62例ESCC及其相应正常食管黏膜组织中HIF-1α和AQP1蛋白的表达。结果:ESCC组织中HIF-1α蛋白阳性表达率为80.6%(50/62),高于正常食管黏膜组织中的19.4%(12/62)(χ2=30.083,P<0.001),并与ESCC组织学分级、TNM分期和淋巴结转移有关(χ2=9.939、6.681和5.787,P均<0.05);ESCC组织中AQP1蛋白阳性表达率为61.3%(38/62),高于正常食管黏膜组织中的38.7%(24/62)(χ2=4.900,P=0.029),与ESCC组织学分级、TNM分期和淋巴结转移有关(χ2=9.102、9.836和8.020,P均<0.05);ESCC组织中HIF-1α与AQP1蛋白的表达有关联(rP=0.292,P=0.016)。结论:高表达的HIF-1α和AQP1与ES-CC的发生发展有关。Aim：To investigate the significance of hypoxia-inducible factor（HIF）-1α and aquaporin（AQP） 1 proteins expression in esophageal squamous cell carcinoma（ESCC） tissue.Methods：The expressions of HIF-1α and AQP1 in 62 samples of ESCC and the corresponding normal esophageal mucosal epithelium tissue were assessed using immunohistochemistry method.Results：The positive expression rates of HIF-1α and AQP1 were 80.6%（50/62） and 61.3%（38/62） in ESCC tissue,and 19.4%（12/62） and 38.7%（24/62） in the normal esophageal mucosa epithelium tissue.The positive expression rates of HIF-1α and AQP1 in ESCC tissue were higher than those in the normal tissue（χ2=30.083,4.900,P0.05）.The expression of HIF-1α was associated with the tumor histological grade,TNM classification and lymph node metastasis of ESCC（χ2=9.939,6.681,5.787,P0.05）,and so was AQP1（χ2=9.102,9.836,8.020,P0.05）.The expression of HIF-1α was associated with the expression of AQP1（rP=0.292,P=0.016） in ESCC tissue.Conclusion： The over-expression of HIF-1α and AQP1 may be involved in carcinogenesis and development of ESCC.

关 键 词：水通道蛋白1 缺氧诱导因子1Α 食管肿瘤 鳞状细胞癌 

分 类 号：R735.1[医药卫生—肿瘤]