顺铂对TRAIL诱导EC1细胞凋亡的增敏效应  

Sensitive enhancement of cisplatin on TRAIL-mediated apoptosis in EC1 cells

作　　者：江亚南[1] 马俊芬[1] 黄幼田[1] 李沛[1] 李芳芳[2] 杨浩[2] 董子明[1] 

机构地区：[1]郑州大学基础医学院病理生理学教研室,郑州450001 [2]郑州大学基础医学院临床医学系,郑州450001

出　　处：《郑州大学学报（医学版）》2011年第3期346-348,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：郑州大学学生创新实验基金资助项目2009qnz007

摘　　要：目的:观察顺铂(cDDP)对肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导EC1细胞凋亡的增敏效应。方法:MTT法检测单用TRAIL(10、50、100、500和1000μg/L)或cDDP(1.0、2.0、4.0、10.0和20.0mg/L)分别作用24、48和72h对EC1细胞增殖的影响。流式细胞术检测100μg/LTRAIL和10.0mg/LcDDP单用或联用48h对EC1细胞凋亡和细胞周期的影响,并设空白对照组。结果:单用TRAIL作用24、48和72h对EC1细胞增殖影响不明显(P>0.05),单用cDDP对EC1细胞增殖有抑制作用(P<0.05)。单用TRAIL对EC1细胞凋亡率无影响(FTRAIL=5.836,P=0.250),而单用cDDP可提高EC1细胞凋亡率(FcDDP=20.763,P=0.041),二者联合有交互作用(F交互=57.422,P<0.001)。单用TRAIL和cDDP对EC1细胞周期无影响,二者联用后G0/G1和S期细胞比例下降,G2/M细胞比例上升(P均<0.001)。结论:cDDP可增加EC1细胞对TRAIL的敏感性,二者联用可诱导EC1细胞凋亡,并诱导其在G/M期阻滞。Aim：To investigate the sensitive enhancement of cisplatin（cDDP） on tumor necrosis factor-related apoptosis-inducing ligand（TRAIL）-mediated apoptosis in esophageal cancer EC1 cells.Methods：The effects of TRAIL （10,50,100,500,and 1 000 μg/L） or cDDP（1.0,2.0,4.0,10.0,and 20.0 mg/L） on the cell proliferation of EC1 for 24,48 and 72 h were observed by MTT.EC1 cells were allocated into 4 groups and treated with 100 mg/L TRAIL or 100 mg/L cDDP or combined use of the 2 drugs for 48 h,and the cells not being treated were control.The apoptosis and cell cycles of EC1 were measured by flow cytometry.Results：TRAIL did not apparently influence the proliferation of EC1 cells（P0.05）,however,CDDP inhibited the proliferation of EC1 cells（P0.05） after 24,48 and 72 h treatment.TRAIL had no influence on the cell apoptosis rate of EC1（FTRAIL=5.836,P=0.250）,while cDDP could increase the cell apoptosis rate（FcDDP=20.763,P=0.041）.Furthermore,the cell apoptosis rate was greatly increased when the 2 drugs were combined（Fboth=57.422,P0.001）.TRAIL or cDDP single had no effects on cell cycle,while combined use of the 2 drugs decreased the percentages of G0/G1 and S phase cells and increased the percentage of G2/M phase cells（P0.05）.Conclusion：cDDP has sensitive enhancement on TRAIL-mediated apoptosis in EC1 cells.TRAIL in combination with cDDP induces EC1 apoptosis and cell cycle to arrest at G2/M phase.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 顺铂 EC1细胞 细胞凋亡 细胞周期 

分 类 号：R735.1[医药卫生—肿瘤]