纳米脂质体槲皮素联合8-Br-cAMP对Rb44细胞凋亡的影响  

Effects of nanoliposomal quercetin combined with 8-Br-cAMP on apoptosis of Rb44 cells

作　　者：郭慧丽[1] 张明昌[1] 郑乃刚[2] 吴景兰[2] 王爱凤[3] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430032 [2]郑州大学分子细胞生物学研究中心,郑州450001 [3]河南省人民医院眼科,郑州450003

出　　处：《郑州大学学报（医学版）》2011年第3期393-395,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技厅基础与前沿技术研究计划基金资助项目102300410025

摘　　要：目的:探讨纳米脂质体槲皮素(nLQ)和8-Br-cAMP(Br)对人视网膜母细胞瘤Rb44细胞凋亡和p53、p21waf1mRNA表达及Bcl-2、Bax和Caspase-3蛋白表达的影响。方法:Rb44细胞分为4组:nLQ组给予终浓度为40μmol/LnLQ;Br组给予终浓度为2×10-5mol/LBr,联合用药组给予同上浓度的nLQ和Br,对照组不给药。采用TUNEL法检测各组细胞的凋亡率。采用原位杂交技术检测p53、p21waf1mRNA的表达,免疫细胞化学方法检测Bcl-2、Bax及Caspase-3蛋白的表达。结果:与对照组相比,单用nLQ或Br均可增高凋亡率,上调p53和p21waf1mRNA的表达及Bax和Caspase-3蛋白的表达,下调Bcl-2蛋白的表达(P<0.001);nLQ及Br联用呈现协同效应(P<0.001)。结论:nLQ和8-Br-cAMP可能通过上调p53和p21waf1基因的表达,诱导线粒体途径和Caspase-3的最终共同凋亡途径而诱导Rb44细胞趋向凋亡,且两药联用呈现协同效应。Aim：To explore the synergistic effects of the nanoliposomal quercetin（nLQ） and 8-Br-cAMP（Br） on apoptosis and expressions of p53 mRNA,p21waf1 mRNA,Bcl-2,Bax and Caspase-3 in Rb44 cells.Methods：Rb44 cells were allocated into 4 groups：nLQ group （given 40 μmol/L nLQ）,Br group （given 2×10-5 mol/L Br）,combined group（given the 2 drugs）,and control group （not treated）.The apoptosis was detected by TUNEL.The mRNA expressions of p53 and p21waf1 were examined by in situ hybridization.The expressions of Bcl-2,Bax and Caspase-3 were detected by immunocytochemistry.Results：Compared with control group,the apoptotic rate increased,the mRNA expressions of p53 and p21waf1 was upregulated,the expression of Bcl-2 was down-regulated,and those of Bax and Caspase-3 were promoted in nLQ and Br groups（P0.001）.Moreover,nLQ and Br combined use had synergistic effects （P0.001）.Conclusion：nLQ and Br could induce apoptosis with synergistic effects through mitochondria and final common apoptotic pathway.

关 键 词：凋亡 纳米脂质体槲皮素 8-BR-CAMP Rb44细胞 

分 类 号：R739.703[医药卫生—肿瘤]