人脑胶质瘤中p16、p15基因分子的病理学研究  

MOLECULAR PATHOLOGICAL STUDY ON p15, p16 GENE IN HUMAN GLIOMAS

作　　者：江澄川[1] 田新华[2] 高翔[1] 陈商群[1] 徐凯黎 周瑾[3] 

机构地区：[1]上海医科大学华山医院神经外科,200040 [2]山西医科大学第二医院神经外科,030001 [3]上海市肿瘤研究所,200032

出　　处：《中国临床神经科学》1999年第4期187-189,193,共4页Chinese Journal of Clinical Neurosciences

基　　金：本课题为国家教委博士点基金资助(96026515)

摘　　要：目的：探讨ｐ１６、ｐ１５基因改变、蛋白表达与脑胶质瘤组织病理的相互关系。方法：应用ＰＣＲ、银染ＰＣＲ－ＳＳＣＰ及免疫组化技术检测６３例脑胶质瘤中ｐ１６、ｐ１５基因变化和蛋白表达情况。结果：显示脑胶质瘤中ｐ１６１、ｐ１５基因缺失和Ｐ１６蛋白丢失主要见于Ⅲ￣Ⅳ级肿瘤中，而ＰＣＲ－ＳＳＣＰ分析凶ｐ１６、ｐ１５基因点突变。结论：表明脑胶质中ｐ１６、ｐ１５基因改变是以缺失为主。Aim: To evaluate the changes of p16, p15 gene, P16 protein expression in human gliomas and relationship between p16,p15 gene and the clinical pathology. Methods: The 68 cases of brain gliomas were detected by PCR, silver-staining PCR-SSCP and immunohistochemistry. Results: Loss of pl6,p15 gene and P16 protein were primarily occurred among the grade Ⅲ and grade Ⅳ of gliomas. From all samples mutations of p16, p15 genes were not found. Conclusion: Deletion of pl6,p15 gene was a more efficient and common mechanism for simultaneous inactivation. The loss of p16,p15 gene is associated with the histological types and progression. The state of P16 protein expression can be regarded as a marker for the differentiation and prognosis of human gliomas.

关 键 词：脑胶质瘤 基因表达 P16基因 P15基因 

分 类 号：R739.410.2[医药卫生—肿瘤]