百日咳菌液对大鼠培养脑切片兴奋性氨基酸释放的影响  

Effect of pertussis bacilli on the release of excitatory amino acids in brain slice of rats

作　　者：尹飞 杨于嘉 虞佩兰 毛定安 

出　　处：《湖南医科大学学报》1999年第6期510-512,共3页Bulletin of Hunan Medical University

基　　金：国家自然科学基金!资助课题(编号:39470233)

摘　　要：目的:了解百日咳菌液对培养的大鼠脑切片兴奋性氨基酸(EAAs) 释放的影响及11 双氢5H二苯(a ,d)环庚烯亚胺( MK801) 对脑切片有无保护作用。方法:SD 大鼠随机分成正常脑切片培养组(NC组) ,10 % 百日咳菌液脑切片培养组(PBC 组) 和MK801 预处理后10 % 百日咳菌液脑切片培养组( MPBC组) 。用高效液相色谱法测脑切片培养上清液中的EAAs 释放量。结果:PBC 组的谷氨酸(Glu) 、门冬氨酸(Asp) 及乳酸脱氢酶(LDH) 的释放量明显高于MPBC 组及NC 组。结论:百日咳菌液可使培养的脑切片组织EAAs 和LDH 的释放量增多;MK801 预处理可降低脑切片组织EAAs 及LDH 的释放量。提示MK801Objective: To investigate the effect of pertussis bacilli on the release of excitatory amino acids(EAAs) in rat brain slice and to know whether MK 801(dizocipine) protects brain tissue from excitotoxicity. Methods: SD rats were randomly divided into 3 groups: ①Normal slice cultured group(NC); ②10% pertussis bacilli(PB) slice cultured group(PBC); ③10% PB slice cultured group after pretreatment with MK 801. The release of EAAs from brain slices was measured by high performance liquid chromatography. Results: The concentrations of glutamate(Glu 29.53±8.00), aspartate(Asp 23.74±5.79) and LDH(38.03±8.45) were significantly higher in PBC group than those(Glu 16.46±4.00, Asp 15.27±5.31, LDH 23.10±5.11) in MPBC group and (Glu 16.09±3.74, Asp 11.47±2.51, LDH 25.82±4.50) NC group. Conclusion: Pertussis bacilli may elicit a large increase of extracellular EAAs and LDH in vitro, while MK 801 protects brain tissue from excitotoxicity.

关 键 词：百日咳杆菌 氨基酸 MK-801 大鼠 脑水肿 

分 类 号：R742.7[医药卫生—神经病学与精神病学] R378.42[医药卫生—临床医学]