nm23-H_1基因mRNA在人肝细胞癌中的表达  

Expression of nm23 H_1 mRNA in hepatocellular carcinoma

作　　者：徐骁[1] 郑树森[1] 陈智[2] 吴灵娇[2] 梁庭波[1] 黄东胜[1] 

机构地区：[1]浙江医科大学附属第一医院肝胆外科,杭州市310003 [2]浙江医科大学传染病研究所

出　　处：《中华肝胆外科杂志》1999年第6期399-401,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的　研究人肝细胞癌中肿瘤转移抑制基因ｎｍ２３Ｈ１ 的ｍＲＮＡ表达状况，并探讨其表达水平与肿瘤各项临床病理指标间的相关性。方法　应用半定量ＲＴＰＣＲ 方法检测分析人肝细胞癌组织、相应癌旁肝组织中ｎｍ２３Ｈ１ 的ｍＲＮＡ表达。结果　１５ 例肝癌及癌旁肝组织ＲＴＰＣＲ结果均为阳性，未见ｎｍ２３Ｈ１ 基因表达缺失或较大变异；肝癌组织中ｎｍ２３Ｈ１ ｍＲＮＡ表达水平明显高于相应癌旁组织（ Ｐ＝ ０－０３５）；ＡＦＰ阳性组ｎｍ２３Ｈ１ ｍＲＮＡ表达水平明显高于ＡＦＰ阴性组（ Ｐ＝０－００５） 。结论　人肝细胞癌中ｎｍ２３Ｈ１ 基因ｍＲＮＡ的异常表达可能与肿瘤的恶性生物学特征密切相关。Objective To investigate the expression of nm23 H 1 mRNA in human hepatocellular carcinoma (HCC) and the relationship between nm23 H 1 mRNA expression and clinico pthological parameters. Method nm23 H 1 mRNA levels were assessed in matched tumorous and corresponding nontumorous liver samples with the use of a semi quantitative reverse transcription polymerase chain reaction (RT-PCR) procedure. Results Fifteen cases of HCC showed positive expression of nm23 H 1 mRNA, indicating there was no genetic expression loss or obvious variation; nm23 H 1 mRNA levels in HCC were significantly higher than in nontumorous samples (P=0.035); nm23 H 1 mRNA levels in AFP(+) group were significantly higher than in AFP(-) one (P=0.005). Conclusions Abnormal expression of nm23 H 1 mRNA in tumor may be closely associated with the malignant biological characteristics of HCC.

关 键 词：肝细胞肿瘤 NM23-H1基因 mRNA RT-PCR 

分 类 号：R735.702[医药卫生—肿瘤]