ALCAM在食管癌中的表达及其功能研究  

A Study of the Expression and Function of ALCAM in Esophageal Cancer

作　　者：孙力超[1] 李智峰[1] 李闯[1] 韩璐璐[1] 杨治华[1] 冉宇靓[1] 

机构地区：[1]中国医学科学院北京协和医学院肿瘤研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《中国肿瘤》2011年第6期447-452,共6页China Cancer

基　　金：国家重点基础研究发展计划(973)资助项目(2009CB521804)

摘　　要：[目的]研究ALCAM基因在食管癌及其淋巴结转移灶中的表达,以及ALCAM在淋巴细胞内皮黏附、跨淋巴内皮迁移中的作用。[方法]免疫组织化学法检测ALCAM基因在食管癌中的表达;应用Lipofectin2000将ALCAM-si-RNA转染到人食管癌细胞株KYSE30、EC9706;采用RT-PCR方法检测敲降效率;通过体外与淋巴内皮细胞黏附实验、跨淋巴内皮迁移实验研究ALCAM基因对食管癌与淋巴内皮细胞黏附、跨内皮的影响。[结果]ALCAM在食管癌组织以及淋巴结转移灶中的表达显著高于正常食管组织。ALCAM-siRNA能显著敲降EC9706中ALCAM的表达。淋巴内皮黏附实验结果显示,敲降ALCAM的食管癌细胞EC9706与LyEC黏附的细胞数为195±16,而亲本细胞的黏附细胞数为563±67,其与LyEC黏附的能力显著下降。跨淋巴内皮实验结果显示敲降ALCAM的EC9706细胞跨过LyEC数量为36±17,与亲本细胞跨过内皮的细胞数(73±16)相比,敲降ALCAM的食管癌细胞的跨内皮能力显著下降。[结论]ALCAM促进食管癌细胞与淋巴细胞内皮黏附以及跨内皮迁移,可能与食管癌的淋巴结转移相关,阻断这种黏附作用有可能治疗食管癌的淋巴结转移。[Purpose] To study the expression of ALCAM in esophageal cancer coupled with lymphatic metastatic foci tissues and the effect of ALCAM gene in esophageal cancer cells on adhesion with lymphatic endothelial cells（LyEC） and transendothelial migration.[Methods] The expression of ALCAM in esophageal cancer tissues was detected by immunochemistry.Human esophageal cancer cell lines KYSE30 and EC9706 were transfected with siRNA-ALCAM or NC-control by Lipofectin 2000.The knockdown efficiency of siRNA was tested by RT-PCR.The adhesive ability with LyEC was tested by adhesive assay and transendothelial ability was tested using Transwell.[Results] The expression of ALCAM in esophageal cancer and lymphatic metastatic foci tissues was considerably higher than that in normal esophageal tissues.The SiRNA-ALCAM could significantly suppress the expression of ALCAM in EC9706.Adhesion with LyEC experiment showed that silence of ALCAM in EC9706 cells significantly inhibited the adhesion with LyEC.The adhesive cell numbers were 195±16 for EC9706 ALCAM knockdown cells and 563±67 for parent cells.Transendothelial assay showed that knockdown of ALCAM in esophageal cancer cells （36±17） decreased significantly compared with parent cells（73±16）.[Conclusions] ALCAM could promote esophageal cancer cell adhesion with LyEC and transendothelial migration,which may be a potential target of the treatment for esophageal cancer lymphatic metastasis.

关 键 词：ALCAM 食管癌 淋巴细胞内皮 黏附 跨内皮迁移 

分 类 号：R735.1[医药卫生—肿瘤]