人涎腺导管癌细胞培养的初步研究  

Cultivation and Biological Characteristics of Primary of Human Salivery Duct Carcinoma Cells

作　　者：王卫红[1] 王春艳[2] 牟象鑫[1] 谢玲[1] 许彪[1] 

机构地区：[1]昆明医学院附属口腔医院口腔颌面外科,云南昆明650031 [2]昆明医学院第一附属医院病理科

出　　处：《口腔医学研究》2011年第6期460-463,共4页Journal of Oral Science Research

基　　金：云南省科技厅-昆明医学院联合专项基金(编号:2007C0030R)

摘　　要：目的:通过体外培养涎腺导管癌细胞,为进一步研究治疗涎腺导管癌奠定基础。方法:对原发于腮腺部位的导管癌进行体外培养。观察细胞生长状况,并通过HE染色、流式细胞术(FCM)及免疫细胞化学检测细胞内细胞角蛋白(CK)、巨囊性病液体蛋白(GCDFP-15)、雄激素受体(AR)特异性抗原标记物的表达,对涎腺导管癌进行形态学检查和鉴定。结果:涎腺导管癌体外培养细胞呈多边形,不规则,有突起,异倍体明显。细胞的生长曲线有明显快速生长期。肿瘤性细胞CK、GCDFP-15、AR阳性表达。结论:涎腺导管癌细胞与原发肿瘤的组织学特征相似。Objective： To cultivate the salivary duct carcinoma（SDC） cell in vitro so as to facilitate forward studying and treating of SDC.Methods： The sample was obtained from a female patient with primary duct carcinoma in parotid gland.We observed the morphologic features of SDC cells and identified SDC cells by using HE staining,FCM and immunocytochemistry staining including cytokeratin（CK）,GCDFP-15 and androgen receptor（AR） respectively.Results： HE staining showed that SDC cells were polygonal shapes,irregular evection and obvious polyploid in vitro.FMC showed that the growth curve had a quick growth phase.Immunocytochemistry staining indicated that the antibody CK,GCDFP-15 and AR was labelled in the SDC cells.Conclusion： The infinite cell of salivary duct carcinoma had been established,and it maintained the properties of primary SDC cells.

关 键 词：导管癌 涎腺 细胞培养 

分 类 号：R739.8[医药卫生—肿瘤]