ICAM-1和VEGF在KK^(-AY)糖尿病小鼠视网膜中的表达  

Expression of ICAM-1 and VEGF in the retina of KK^(-AY) diabetic mice

作　　者：王巍[1] 张晓梅[1] 王彬杰[1] 崔浩[1] 

机构地区：[1]哈尔滨医科大学附属第一医院眼科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2011年第3期222-225,共4页Journal of Harbin Medical University

基　　金：哈尔滨医科大学附属第一医院科研基金项目(2009Y03)

摘　　要：目的探讨KK-AY糖尿病小鼠视网膜中细胞间黏附分子-1(ICAM-1)和血管内皮细胞生长因子(VEGF)表达情况。方法实验共分为4组,每组10只小鼠。对照组为12周的C57BL/6小鼠;糖尿病模型组为KK-AY小鼠,分别为12周、16周、20周组。测量各实验组体重、血糖,并应用逆转录聚合酶链式反应(RT-PCR)和蛋白免疫印迹(Western blot)方法检测视网膜中ICAM-1和VEGF mRNA和蛋白的表达情况。结果糖尿病模型组12周时视网膜ICAM-1和VEGF表达均出现上升,16周时继续增加,20周时分别到达其最高点,其中16周、20周组与对照组相比差异均有统计学意义(P<0.05)。结论 ICAM-1和VEGF可能参与KK-AY小鼠糖尿病视网膜病变的发生。Objective To study the expression of intercellular adhesion molecule-1（ICAM-1） and vascular endothelial growth factor（VEGF） in retina of KK-AY mice.Methods Experimental mice were divided into 4 groups： C57BL/6（control group） and KK-AY diabetic mice groups（12-week,16-week,20-week group）,10 mice in each group.The body weight and blood glucose of mice were measured,meanwhile,the expression of ICAM-1 and VEGF in each group was observed using reverse transcription-polymerase chain reaction（RT-PCR） and western blot analysis.Results The expression of ICAM-1 and VEGF increased in 12-week KK-AY mouse retina,with higher expression in 16-week group,and had the highest expression in 20-week group.The difference between the control group and the 16-week,20-week groups was significant（P0.05）.Conclusion ICAM-1 and VEGF may participate in the development of diabetic retinopathy.

关 键 词：KK-AY小鼠 糖尿病视网膜病 细胞间黏附分子-1 血管内皮细胞生长因子 

分 类 号：R774.1[医药卫生—眼科]