突变体c-myc对平滑肌细胞迁移和侵袭的调控  

Regulating on migration and invasion of vascular smooth muscle cells by mutant c-myc

作　　者：卢群[1] 罗少洪[1] 何伟彬[1] 温永芳[1] 

机构地区：[1]广东药学院基础学院,广东广州510006

出　　处：《广东药学院学报》2011年第3期309-311,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金(8451022401000547);广东省科技计划项目(2010B031600292)

摘　　要：目的研究突变体c-myc(T58A、S62A)、WTc-myc对平滑肌细胞迁移和侵袭的调控。方法平滑肌细胞转染T58A、S62A、WT后采用Western blot检测法、荧光探针检测法、细胞迁移和侵袭检测法,测c-myc的表达、转录活性及平滑肌细胞迁移和侵袭的程度。结果平滑肌细胞转染T58A、S62A、WT后c-myc蛋白表达显著增加;转录活性增强;平滑肌细胞迁移和侵袭增加,T58A的促进作用比S62A强。结论 T58A、S62A和WTc-myc明显诱导平滑肌细胞c-myc表达增加,促进平滑肌细胞迁移和侵袭,T58A为主要调控靶点。Objective To investigate the effect of mutant c-myc on vascular smooth muscle cells（VSMC） migration and invasion.Methods Western blot analysis,luciferase assay,and VSMC migration and invasion assay were used to determine c-myc protein expression,c-myc transcriptional activity,and VSMC migration and invasion ability.Result c-myc protein levels and transcriptional activity were significantly increased in VSMC when transfected with mutant c-myc（T58A,S62A） or wild type（WT） c-myc gene.VSMC migration and invasion ability was also greatly enhanced after tranfected with c-myc gene.The effect of T58A was more active than S62A.Conclusion WT,T58A and S62A c-myc genes significantly induce c-myc protein expression and promote VSMC migration and invasion,and T58A especially takes on an important function.

关 键 词：T58A S62A WT c—myc 平滑肌细胞 迁移 侵袭 调控 

分 类 号：R363[医药卫生—病理学]