基于核磁共振波谱的鼻咽癌患者血清代谢组研究  

^1H NMR spectroscopy combined with partial least square-discriminant analysis for metabonomic study on serum of NPC patients

作　　者：符方方[1] 姜苗苗[1] 王希成[2] 张宇[1] 冯毅凡[1] 

机构地区：[1]广东药学院分析测试中心,广东广州510006 [2]广东药学院第一附属医院肿瘤科,广东广州510006

出　　处：《广东药学院学报》2011年第3期312-315,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省医学科学技术研究基金(B2010148)

摘　　要：目的应用核磁共振氢谱(nuclearmagnetic resonance1H,1H NMR)和偏最小二乘法-判别分析(partialleast square-discriminant analysis,PLS-DA)研究鼻咽癌(NPC)患者血清中代谢物的代谢组变化。方法健康人血清10例和鼻咽癌患者血清21例,采用BRUKER 500 MHz超导核磁共振波谱仪进行检测,并运用PLS-DA分析方法,得出鼻咽癌患者血清代谢组的特征,探讨相关生化过程的改变。结果鼻咽癌患者血清中的丙氨酸、异亮氨酸、亮氨酸、苯丙氨酸、脂类和不饱和脂肪酸等含量升高,而乳酸、缬氨酸、谷氨酰胺以及胆碱类物质降低,与正常人血清代谢组比较差异有统计学意义(P<0.05)。结论基于核磁共振氢谱和偏最小二乘法-判别分析的代谢组学方法可为鼻咽癌的诊断提供分子水平上的代谢依据。Objective To characterize the metabolite composition（ie,metabonome） of the serum samples from nasopharyngeal cancer patients by using 1H NMR（nuclear magnetic resonance1H） spectroscopy combined with partial least square-discriminant analysis（PLS-DA）.Methods The1H NMR sepctra were obtained for the serum samples from 10 healthy controls and 21NPC patients,and the spectra data were analyzed by PLS-DA to demonstrate the metabonomic changes.Results There were evident differences in metabonomics between the healthy controls and NPC patients.The levels of alanine,isoleucine,leucine and phenylalanine were significantly increased in the NPC patient and the levels of glutamine,lactate,and valine were significantly decreased compared with the healthy controls（P〈0.05）.Conclusion The metabonomes of serum from NPC patients are different from those of the healthy controls.Serum 1H NMR sepctroscopy combined with PLS-DA may offer reliable biochemistry evidence for diagnosis of NPC patients at molecular level.

关 键 词：鼻咽癌 血清 代谢物组 核磁共振 模式识别 

分 类 号：R739.63[医药卫生—肿瘤]