肾素基因G10631A和血管紧张素原基因T704C多态性与脑梗死的关系  

Relationship between renin gene G10631A, T704C polymorphism of angiotensinogen gene and cerebral infarction

作　　者：孙洪英[1] 和姬苓[1] 杨玉蓉[1] 张佳[2] 张利荣[3] 

机构地区：[1]包头医学院第一附属医院神经内科,014010 [2]包头医学院,014040 [3]包头医学院第一附属医院影像中心,014010

出　　处：《国际脑血管病杂志》2011年第6期442-446,共5页International Journal of Cerebrovascular Diseases

基　　金：内蒙古自治区卫生厅项目（2005030）

摘　　要：目的探讨肾素（renin，REN）基因G10631A和血管紧张素原（anbotensinogen，AGT）基因T704C单核苷酸多态性与脑梗死的关系，从分子水平探讨脑梗死发病的机制及特点。方法采用病例对照研究设计，通过聚合酶链反应-限制性片段长度多态性技术，检N82例脑梗死患者和89例对照者RENG10631A和AGTT704C多态性，比较病例组与对照组基因型和等位基因频率的差异。结果脑梗死组REN10631AA基因型频率（31．7％对10．1％，χ2=12．816，P=0．002）和A等位基因频率（49．4％对30．3％,χ2=12．969，P=0．000）以及AGT704CC基因型频率（63．4％对34．8％,χ2=15．029，P=0．001）和C等位基因频率（79．9％对61．2％，χ2=14．173，P=0．000）均显著高于对照组；单倍型704C-10631A频率也显著高于对照组（P=0．001）。结论REN10631AA基因型和A等位基因以及AGT704CC基因型和C等位基因可能为脑梗死的易感因素，单倍型704C-10631A可能是脑梗死发病的遗传危险因素。Objective To investigate the relationship between renin （REN） genne G10631A, angiotensinon （AGT） gene T704C mononucleotide polymorphisms and cerebral infarction and to investigate the mechanisms and characteristics of cerebral infarction from molecular level. Methods REN gene G1063A and AGT gene T704C polymorphisms in 82 patients with cerebral infarction and 89 controls were detected with polymerase chain reaction- restriction fragnent length polymorphism. The differences of the genotypes and allele frequencies were compared between the patient group and the control group. Results The frequency of REN 10631AA genotype （31.7% vs. 10. 1% ,χ2 =12. 816, P =0. 002） and the frequency of A genotype （49. 4% vs. 30. 3%χ2 = 12. 969, P =0. 000）, as well as the frequency of AGT 704 CC genaotype （63.4% vs. 34. 8% χ2 = 15. 029, P = 0. 001） and the frequency of A genotype （79. 9% vs. 61.2% χ2 = 14. 173, P =0. 000） in the cerebral infarction group were all signifi- cantly higher than those in the control group; the frequency of haplotype 704C 10631A was also significantly hiher than that in the control group （P =0. 000）. Conclusions REN 10631AA genetype and A allele as well as AGT 704 CC genaetype and C allele may be the susceptible factors of cerebral infarction. Haplotype 704C-10631 A may be a genetic risk factor for the occurrence of cerebral infarction.

关 键 词：脑梗死 卒中 肾素 血管紧张素原 多态现象 遗传学 单元型 

分 类 号：R74[医药卫生—神经病学与精神病学]